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Geldanamycin selectively targets the nascent form of ERBB3 for degradation

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Cell Stress and Chaperones Aims and scope

Abstract

Heat shock protein 90 (HSP90) targets a broad spectrum of client proteins with divergent modes of interaction and consequences. The homologous epidermal growth factor receptor (EGFR) and ERBB2 receptors as well as kinase-deficient mutants thereof differ in their requirement for HSP90 in the nascent versus mature state of the receptor. Specific features of the kinase domain have been implicated for the selective association of HSP90 with mature ERBB2. We evaluated the role of HSP90 for the homologous ERBB3 receptor. ERBB3 is naturally kinase deficient, a central mediator in cell survival and stress response and the primary dimerization partner for ERBB2 in signaling. Cellular studies indicate that, similar to EGFR, the geldanamycin (GA) sensitivity of ERBB3 and HSP90 binding resides in the nascent state and is dependent on the presence of the kinase domain of ERBB3. Furthermore, despite its intrinsic lack of kinase activity and in contrast to the reported GA sensitivity of mature and kinase-deficient EGFR, the GA sensitivity of the nascent state of ERBB3 appears to be exclusive. Geldanamycin disrupts the interaction of ERBB3 and HSP90 and inhibits ERBB3 maturation at an early stage of synthesis, prior to export from the ER. Studies with a photo-convertible fusion protein of ERBB3 suggest geldanamycin sensitivity at a later stage in maturation, possibly through the putative role of HSP90 in structural proofreading.

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Acknowledgements

We thank Dr. Walter Scott for critical reading and suggestions during the writing of this manuscript and members of the Shuai lab at UCLA for help with qPCR analysis. This work was supported by funding from the National Institutes of Health (RL, CA098881-01A1, CA98881-05).

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, University of Miami, Coral Gables, FL, USA

    Ralf Landgraf

  2. Department Medicine, Division of Hematology-Oncology, University of California Los Angeles, Los Angeles, CA, USA

    Ralf Landgraf

  3. Department of Biological Chemistry, University of California Los Angeles, Los Angeles, CA, USA

    Candice S. Gerbin

  4. Dept. of Biochemistry and Molecular Biology, Miller School of Medicine, Box 01629 (R-629), Miami, FL, 33101-6129, USA

    Ralf Landgraf

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  1. Candice S. Gerbin
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  2. Ralf Landgraf
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Correspondence to Ralf Landgraf.

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This work was supported by funding from the National Institutes of Health (RL, CA098881).

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Supplementary Fig. 1

Ribbon diagram and surface charge representation of the kinase domains of EGFR in the active conformation (1M17, Stamos et al. 2002) and lapatinib-stabilized inactive conformation (1XKK, Wood et al. 2004). Large segments (outlined in purple) are not resolved in the inactive conformation, making surface charge projections of this portion of the kinase domain inaccurate. However, the area around the αC-β4 loop is resolved and retains the characteristic negative surface charge projection, associated with a failure of mature EGFR to bind HSP90. (JPEG 4674 kb)

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Gerbin, C.S., Landgraf, R. Geldanamycin selectively targets the nascent form of ERBB3 for degradation. Cell Stress and Chaperones 15, 529–544 (2010). https://doi.org/10.1007/s12192-009-0166-1

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  • DOI: https://doi.org/10.1007/s12192-009-0166-1

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