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Ingestion of bacteria overproducing DnaK attenuates Vibrio infection of Artemia franciscana larvae

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Cell Stress and Chaperones Aims and scope

Abstract

Feeding of bacterially encapsulated heat shock proteins (Hsps) to invertebrates is a novel way to limit Vibrio infection. As an example, ingestion of Escherichia coli overproducing prokaryotic Hsps significantly improves survival of gnotobiotically cultured Artemia larvae upon challenge with pathogenic Vibrio campbellii. The relationship between Hsp accumulation and enhanced resistance to infection may involve DnaK, the prokaryotic equivalent to Hsp70, a major molecular chaperone in eukaryotic cells. In support of this proposal, heat-stressed bacterial strains LVS 2 (Bacillus sp.), LVS 3 (Aeromonas hydrophila), LVS 8 (Vibrio sp.), GR 8 (Cytophaga sp.), and GR 10 (Roseobacter sp.) were shown in this work to be more effective than nonheated bacteria in protecting gnotobiotic Artemia larvae against V. campbellii challenge. Immunoprobing of Western blots and quantification by enzyme-linked immunosorbent assay revealed that the amount of DnaK in bacteria and their ability to enhance larval resistance to infection by V. campbellii are correlated. Although the function of DnaK is uncertain, it may improve tolerance to V. campbellii via immune stimulation, a possibility of significance from a fundamental perspective and also because it could be applied in aquaculture, a major method of food production.

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Acknowledgements

This work was supported by the University Malaysia Terengganu (UMT, formerly known as University College of Science and Technology Malaysia, KUSTEM) through a doctoral grant to YYS. Research funding was from the Belgian Foundation for Scientific Research (FWO) through the project “Probiont-induced functional responses in aquatic organisms” (no. G.0491.08) and from the Natural Sciences and Engineering Research Council of Canada to THM. We thank Prof. Dr. Bernd Bukau and Dr. Axel Mogk from the Centrum for Molecular Biology, University Heidelberg, Germany for the generous gift of polyclonal antibody to DnaK.

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Authors and Affiliations

  1. Department of Fisheries and Aquaculture, Faculty of Agrotechnology and Food Science, University Malaysia Terengganu, 21030, Kuala Terengganu, Malaysia

    Yeong Yik Sung

  2. Institute of Tropical Aquaculture (AQUATROP), University Malaysia Terengganu, 21030, Kuala Terengganu, Malaysia

    Yeong Yik Sung

  3. Laboratory of Aquaculture and Artemia Reference Center, Ghent University, Rozier 44, 9000, Ghent, Belgium

    Till Dhaene, Tom Defoirdt, Patrick Sorgeloos & Peter Bossier

  4. Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Coupure Links 653, 9000, Ghent, Belgium

    Nico Boon

  5. Department of Biology, Dalhousie University, Halifax, NS, B3H 4 J1, Canada

    Thomas H. MacRae

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  1. Yeong Yik Sung
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  2. Till Dhaene
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Correspondence to Yeong Yik Sung.

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Sung, Y.Y., Dhaene, T., Defoirdt, T. et al. Ingestion of bacteria overproducing DnaK attenuates Vibrio infection of Artemia franciscana larvae. Cell Stress and Chaperones 14, 603–609 (2009). https://doi.org/10.1007/s12192-009-0112-2

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