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Cloning of cytoplasmic heat shock protein 90 (FcHSP90) from Fenneropenaeus chinensis and its expression response to heat shock and hypoxia

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Cell Stress and Chaperones Aims and scope

Abstract

Heat shock protein 90 (HSP90) works as a multi-functional chaperone and is involved in the regulation of many essential cellular pathways. In this study, we have identified a full-length complementary DNA (cDNA) of HSP90 (FcHSP90) from Chinese shrimp Fenneropenaeus chinensis. FcHSP90 full-length cDNA comprised 2,552 bp, including a 2,181-bp open reading frame encoding 726 amino acids. Both homology analyses using alignment with previously identified HSP90 and a phylogeny tree indicated that FcHSP90 was a cytoplasmic HSP90. Real-time reverse transcription polymerase chain reaction analysis revealed that FcHSP90 was ubiquitously expressed in all the examined tissues but with highest levels in ovary of F. chinensis. FcHSP90 mRNA levels were sensitively induced by heat shock (from 25°C to 35°C) and reached the maximum at 6 h during heat shock treatment. Under hypoxia conditions, FcHSP90 mRNA levels, in both hemocytes and gill, were induced at 2 h and depressed at 8 h during hypoxia stress. The assessment of FcHSP90 mRNA levels under heat shock and hypoxia stresses indicated that the transcription of FcHSP90 was very sensitive to heat shock and hypoxia, so we deduced that FcHSP90 might play very important roles for shrimp to cope with environmental stress.

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Acknowledgements

This work was supported by the National High-Tech Research and Development Program of China (863 program) 2006AA09Z424 and 2006AA10A402, the Major State Basic Research Development Program of China (973 program) 2006CB101804.

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Authors and Affiliations

  1. Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China

    Fuhua Li, Wei Luan, Chengsong Zhang, Jiquan Zhang, Bing Wang, Yusu Xie, Shihao Li & Jianhai Xiang

  2. Graduate School, Chinese Academy of Sciences, Beijing, 100039, China

    Wei Luan, Chengsong Zhang, Yusu Xie & Shihao Li

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  1. Fuhua Li
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  2. Wei Luan
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  8. Jianhai Xiang
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Correspondence to Jianhai Xiang.

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Li, F., Luan, W., Zhang, C. et al. Cloning of cytoplasmic heat shock protein 90 (FcHSP90) from Fenneropenaeus chinensis and its expression response to heat shock and hypoxia. Cell Stress and Chaperones 14, 161–172 (2009). https://doi.org/10.1007/s12192-008-0069-6

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  • DOI: https://doi.org/10.1007/s12192-008-0069-6

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