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Extreme thermotolerance and behavioral induction of 70-kDa heat shock proteins and their encoding genes in honey bees

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Cell Stress and Chaperones Aims and scope

Abstract

Foraging honey bees frequently leave the hive to gather pollen and nectar for the colony. This period of their lives is marked by periodic extremes of body temperature, metabolic expenditure, and flight muscle activity. Following ecologically relevant episodes of hyperthermia between 33°C and 50°C, heat shock protein 70 (Hsp70) expression and hsp70/hsc70-4 activity in brains of nonflying laboratory-held bees increased by only two to three times baseline at temperatures 46–50°C. Induction was undetectable in thoracic–flight muscles. Yet, thorax hsp70 mRNA (but not hsc70-4 mRNA) levels were up to ten times higher in flight-capable hive bees and foraging bees compared to 1-day-old, flight-incapable bees, while brain hsp70/hsc70-4 mRNA levels were low and varied little among behavioral groups. These data suggest honey bee tissues, especially flight muscles, are extremely thermotolerant. Furthermore, Hsp70 expression in the thoraces of flight-capable bees is probably flight-induced by oxidative and mechanical damage to flight muscle proteins rather than temperature.

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Acknowledgments

Thanks to Gene Robinson, Yehuda Ben Shahar, Miguel Corona, Jayson Cutler, Christina Grozinger, Kathy Jez, Rodney Mehring, Karen Pruiett, David Schulz, Kate Shen, April Stetler, and Stephen Roberts for comments and/or assistance. This research was supported by a UNLV New Investigator Award and National Science Foundation grant (IOB-0517635).

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  1. School of Life Sciences, University of Nevada Las Vegas, 4505 Maryland Parkway, Box 454004, Las Vegas, NV, 89154-4004, USA

    Michelle M. Elekonich

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Correspondence to Michelle M. Elekonich.

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Elekonich, M.M. Extreme thermotolerance and behavioral induction of 70-kDa heat shock proteins and their encoding genes in honey bees. Cell Stress and Chaperones 14, 219–226 (2009). https://doi.org/10.1007/s12192-008-0063-z

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