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In vivo imaging of the effect of LPS on arterial endothelial cells: molecular imaging of heat shock protein 60 expression

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Cell Stress and Chaperones Aims and scope

Abstract

Bacterial endotoxins are known as stress factors for endothelial cells. In 20 normocholesterolemic New Zealand White (NZW) rabbits, endothelial stress was induced by intravenous (i.v.) injection of lipopolysaccharide (LPS), while eight NZW rabbits were sham-treated or served as untreated controls. In vivo molecular imaging was performed using co-registered computer tomography and positron emission tomography 24 h after i.v. injection of 124I-labeled monoclonal anti-HSP60 or 124I-radiolabelled isotype control antibodies. Compared to control animals, in vivo images of rabbit aortae revealed significantly increased endothelial binding of 124I-labeled anti-HSP60 antibodies upon LPS, especially at sites of aortal branching. This was confirmed by immunohistochemistry and autoradiography data. Our results showed, as proof-of-principle, that HSP60-expression in normocholesterolemic rabbits is significantly increased after induction of endothelial stress and that non-invasive in vivo molecular imaging of early aortal HSP60-expression using 124I-labeled anti-HSP60 monoclonal antibodies is possible.

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Acknowledgements

This work was supported by the Propter Homines Foundation, Vaduz, FL (to GW), the Medizinische Forschungsförderung Innsbruck MFI (to MCW, Project 9443), the European Union as part of the project Molecular Basis of Vascular Events Leading to Thrombotic Stroke (MOLSTROKE; LSHM-CT-2004-005206), and the Network of Excellence European Vascular Genomics Network (EVGN; LSHM-CT-2003-503254). Editorial assistance from M. Kat Occhipinti-Bender is acknowledged.

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Authors and Affiliations

  1. Department of Radiology, Innsbruck Medical University, Anichstrasse 35, 6020, Innsbruck, Austria

    Marius C. Wick & Werner Jaschke

  2. Division of Experimental Pathophysiology and Immunology, Biocenter, Innsbruck Medical University, Fritz-Pregl-Strasse 3, 6020, Innsbruck, Austria

    Christina Mayerl

  3. Division of Experimental Pathophysiology and Immunology, Laboratory of Autoimmunity, Biocenter, Innsbruck Medical University, Fritz-Pregl-Strasse 3, 6020, Innsbruck, Austria

    Aleksandar Backovic & Georg Wick

  4. Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, University of Utrecht, Yalelaan 1, 3584CL, Utrecht, The Netherlands

    Ruurd van der Zee

  5. Central Laboratory Animal Facilities, Innsbruck Medical University, Peter-Mayr-Strasse 4a, 6020, Innsbruck, Austria

    Hermann Dietrich

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  1. Marius C. Wick
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Wick, M.C., Mayerl, C., Backovic, A. et al. In vivo imaging of the effect of LPS on arterial endothelial cells: molecular imaging of heat shock protein 60 expression. Cell Stress and Chaperones 13, 275–285 (2008). https://doi.org/10.1007/s12192-008-0044-2

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  • DOI: https://doi.org/10.1007/s12192-008-0044-2

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