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Multi-Residue Determination of Sulfonamides, Dapsone, Ormethoprim, and Trimethoprim in Fish and Shrimp Using Dispersive Solid Phase Extraction with LC–MS/MS

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Abstract

This study reports an analytical method to simultaneously determine the residues of sulfonamides, dapsone, ormethoprim, and trimethoprim in fish and shrimp. The samples were extracted with acetonitrile, magnesium sulfate, and sodium acetate, followed by dispersive solid-phase extraction (C18) for cleanup. The target analytes were confirmed and quantified by liquid chromatography–tandem mass spectrometry. The developed method was validated according to the Codex guidelines (CAC/GL 71-2009). As a result, linearity was expressed between 0.25 and 200 μg kg−1 with a correlation coefficient > 0.98. The limits of quantitation were 0.01–2.8 μg kg−1 and the accuracy (expressed as average recovery) was 76.1–115%. The precision (expressed as the coefficient of variation) was <20%. The decision limits of sulfonamides, dapsone, ormethoprim, and trimethoprim were 94.2–114, 1.3–1.5, 105–111, and 53.3–54 μg kg−1 and detection capabilities of 102–129, 1.4–1.6, 109–120, and 55.6–58.2 μg kg−1, respectively. The proposed method was applied to the analysis of real samples (n = 54) obtained from domestic markets in Korea, resulting in a detection rate of 13% (seven samples). This method is applicable for the efficient determination of sulfonamides, dapsone, ormethoprim, and trimethoprim residues in fish and shrimp according to Korean MRL.

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Funding

This study was supported by grants (No. 19161MFDS581 and 20161MFDS623) from the Ministry of Food and Drug Safety in South Korea in 2019 and 2020.

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  1. Pesticide and Veterinary Drug Residues Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Osong, Cheongju, Chungcheongbuk-do, 28159, South Korea

    Soo Yeon Choi & Hui-Seung Kang

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  1. Soo Yeon Choi
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SYC: performed experimental work and drafted of manuscript

H-SK: study designed and wrote and revised manuscript

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Correspondence to Hui-Seung Kang.

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SYC and H-SK declare no conflict of interest.

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Choi, S.Y., Kang, HS. Multi-Residue Determination of Sulfonamides, Dapsone, Ormethoprim, and Trimethoprim in Fish and Shrimp Using Dispersive Solid Phase Extraction with LC–MS/MS. Food Anal. Methods 14, 1256–1268 (2021). https://doi.org/10.1007/s12161-021-01965-x

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