Abstract
Yeast extract (YE) is tremendously used as a flavor enhancer in foods because of its composition. However, different types of YE have different aromatic profiles. This study focuses on the thermal treatment of glutathione-enriched yeast extract (GSH-YE) at different temperatures with the addition of Maillard precursors to investigate any change in its volatile organic profile. Headspace gas chromatography-ion mobility spectrometry (HS-GC-IMS) was utilized for the instrumental analysis. A total number of 104 volatile compounds were analyzed through this approach. Qualitative and quantitative analysis of the samples were made in order to calculate the concentration of volatile compounds in samples. Sensory evaluation was performed to compare the overall aroma descriptions with the volatile profile of the heat-treated samples. Sensory scores revealed that an increase in the thermal temperature significantly affected the meaty and roasted aroma of GSH-YE. Many sulfur-containing volatile compounds including 2-methyl-3-furanthiol, 2-furfurylthiol, 2-methyl-3-(methylthio)furan, dimethyl trisulfide, 2-acetylthiazol, and some furans and pyrazines related to meaty and roasted odor notes were reported in this study. Significant increase in the amounts of furfural and dimethyl sulfide was observed in samples having higher thermal temperatures as compared with the control treatment. The temperature of 100 °C with adequate amounts of Maillard precursors in GSH-YE was considered an optimal condition for generating ample amounts of 2-methyl-3-methylthiofuran and 2-furfurylthiol. The 3D graphical data confirms the potential application of HS-GC-IMS as a more potent instrumental approach in the determination of volatile compounds in yeast extract.
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An over view of volatile classification in heat-treated glutathione-enriched yeast extract via GC-IMS technique. A total number of 104 volatile organic compounds were identified through this approach. The concentration of sulfur-containing volatile compounds was significantly changed in three samples.
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Funding
This work was funded by the National Natural Science Foundation of China, under the frame work of Project no. (31571884).
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Ali Raza and Song Huanlu designed this research work. Song Huanlu supervised this research work. Ali Raza conducted this experiment and drafted this manuscript. Nabila begum and Junaid Raza helped in the collection and arrangement of experimental data. Maryam Iftikhar contributed in the editing process. Pei Li and ku Li assisted in designing the treatment plan and experimental analysis.
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Highlights
• A total number of 104 volatile organic compounds were identified in glutathione-enriched yeast extract.
• The highest percentage of meat-like volatile compounds was reported at elevated temperatures.
• The concentration of sulfur-containing volatile compounds was significantly changed in three samples.
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Raza, A., Song, H., Begum, N. et al. Direct Classification of Volatile Organic Compounds in Heat-Treated Glutathione-Enriched Yeast Extract by Headspace-Gas Chromatography-Ion Mobility Spectrometry (HS-GC-IMS). Food Anal. Methods 13, 2279–2289 (2020). https://doi.org/10.1007/s12161-020-01847-8
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DOI: https://doi.org/10.1007/s12161-020-01847-8