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Colorimetric Detection of Horse Meat Based on Loop-Mediated Isothermal Amplification (LAMP)

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Abstract

Meat authentication and meat adulteration have become a growing global concern leading to consumers’ demand for accurate meat identification methods. Since existing methods for meat identification are cumbersome and require specialized equipment and/or training, we developed a specific colorimetric detection method that can rapidly identify horse meat as few as 0.1% contamination in xenogeneic meat sources within 1 h with no false-positive signals observed. Specificity analysis revealed no cross-reactivity with 14 other animal species. Therefore, a rapid on-site survey based on our method can be set up to identify undeclared meat in the food supply chain and contributes to an approach on food analysis by meeting the demands for quick and easy-to-perform analytical methods.

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Abbreviations

FSAI:

Food Safety Authority of Ireland

PBZ:

phenylbutazone

ELISA:

enzyme-linked immunosorbent

mtDNA:

mitochondrial DNA

real-time PCR:

real-time polymerase chain reaction

PCR-RFLP:

PCR-based restriction fragment length polymorphism

LAMP:

loop-mediated isothermal amplification

COI gene:

cytochrome c oxidase subunit I gene

HNB:

hydroxy naphthol blue

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Funding

This work was supported by the National Natural Science Foundation of China [grant numbers 21572222, 21708037, 21877108], the Innovative Team of Sichuan Province [grant number 2017TD0021], Chengdu Municipal Bureau of Science and Technology [grant number 2015-HM02-00099-SF, 2016-HM01-00371-SF], and China Postdoctoral Science Foundation [grant number 2017M612998].

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Authors and Affiliations

  1. Natural Products Research Center, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, People’s Republic of China

    Jiayu Wang, Gangyi Chen, Sheng Ding, Mei Li, Juan Dong, Feng Du, Xin Cui & Zhuo Tang

  2. University of Chinese Academy of sciences, Beijing, 100049, People’s Republic of China

    Jiayu Wang & Zhuo Tang

  3. Chengdu Institute for Food and Drug Control, Chengdu, 610045, People’s Republic of China

    Yuping Wan, Hengxing Liang & Ke Shang

Authors
  1. Jiayu Wang
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  2. Yuping Wan
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  3. Gangyi Chen
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  4. Hengxing Liang
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  5. Sheng Ding
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  6. Ke Shang
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  7. Mei Li
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  8. Juan Dong
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  9. Feng Du
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  10. Xin Cui
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  11. Zhuo Tang
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Corresponding author

Correspondence to Zhuo Tang.

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Conflict of Interest

Jiayu Wang declares that she has no conflict of interest. Yuping Wan declares that she has no conflict of interest. Gangyi Chen declares that he has no conflict of interest. Hengxing Liang declares that he has no conflict of interest. Sheng Ding declares that he has no conflict of interest. Ke Shang declares that he has no conflict of interest. Mei Li declares that she has no conflict of interest. Juan Dong declares that she has no conflict of interest. Feng Du declares that she has no conflict of interest. Xin Cui declares that she has no conflict of interest. Zhuo Tang declares that he has no conflict of interest.

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Wang, J., Wan, Y., Chen, G. et al. Colorimetric Detection of Horse Meat Based on Loop-Mediated Isothermal Amplification (LAMP). Food Anal. Methods 12, 2535–2541 (2019). https://doi.org/10.1007/s12161-019-01590-9

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