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Ultrasound-Assisted Matrix Solid-Phase Dispersion Coupled with Reversed-Phase Dispersive Liquid–Liquid Microextraction for Determination of Vitamin C in Various Matrices

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Abstract

For the first time, ultrasound-assisted matrix solid-phase dispersion (UA-MSPD) and reversed-phase dispersive liquid–liquid microextraction (RP-DLLME) were coupled for determination of vitamin C in various matrices without any filtration and sample transfer from the original vessel. Samples were analyzed using a high-performance liquid chromatography (HPLC) instrument with ultraviolet absorption detector. Influential parameters of UA-MSPD and RP-DLLME such as sorbent, elution solvent and its volume, sample to sorbent ratio, ultrasonic time and amplitude, pH of aqueous phase and its volume, water-immiscible organic solvent and its volume, and centrifuging time were investigated and optimized. Under the optimized conditions, limit of detection and quantitation values were 0.84 and 2.5 ng/mL, respectively. Recovery data for several real samples were obtained in the range of 67.0–88.0% with relative standard deviation (RSD) less than 7.5%. The proposed method was successfully applied to quantitatively determination of vitamin C in several solid and semi-solid samples.

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Acknowledgments

The authors gratefully acknowledge the support of Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad (Iran).

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Authors and Affiliations

  1. Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, 68149-89468, Iran

    Rouhollah Heydari

  2. Department of Chemistry, Semnan University, Semnan, Iran

    Mohammad Reza Darabi Bazvand

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  1. Rouhollah Heydari
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  2. Mohammad Reza Darabi Bazvand
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Correspondence to Rouhollah Heydari.

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Rouhollah Heydari declares that he has no conflict of interest. Mohammad Reza Darabi Bazvand declares that he has no conflict of interest.

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Heydari, R., Darabi Bazvand, M.R. Ultrasound-Assisted Matrix Solid-Phase Dispersion Coupled with Reversed-Phase Dispersive Liquid–Liquid Microextraction for Determination of Vitamin C in Various Matrices. Food Anal. Methods 12, 1949–1956 (2019). https://doi.org/10.1007/s12161-019-01547-y

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