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Clean-up Procedure Development and Method Validation for Pesticide Residues Analysis in Carrots

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Abstract

In this work, a method was developed, validated, and applied to determination of ten pesticide residues (trifluralin, carbofuran, chlorothalonil, pendimethalin, captan, tebuconazole, pyraclostrobin, difenoconazole, deltamethrin, and azoxystrobin), from seven distinct classes, in carrot samples, employing a QuEChERS procedure for the analyte extraction and gas chromatography coupled to mass spectrometry (GC-MS) for quantification. The dispersive clean-up step was optimized using different sorbents at different concentrations and mixings. The method proved to be efficient and robust, showing recoveries in the range 72–117%, and RSD ranging from 4.9 to 16.6% (except for chlorothalonil and captan) at fortification levels of 45, 90, 180, and 450 μg kg−1 with seven replicates analyzed for each level (n = 28). Chlorothalonil and captan could not be validated due to analyte stability problems. Detection limits were between 15 and 45 μg kg−1, and the quantification limits were between 45 and 90 μg kg−1. The method was successfully applied to real samples.

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Acknowledgments

The authors would like to thank to the Federal University of Rio Grande and to the Post Graduate Program in Technological and Environmental Chemistry for free and high quality education, as well as to the National Council for Scientific and Technological Development for the fellowship provided to Juliana L. da S. Batista.

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Authors and Affiliations

  1. Escola de Química e Alimentos, Universidade Federal do Rio Grande, Santo Antônio da Patrulha, RS, 95500-000, Brazil

    Márcia H. S. Kurz, Juliana L. da S. Batista, Lenise G. de Oliveira, Manoel L. Martins & Fábio F. Gonçalves

  2. Laboratório Nacional Agropecuário – LANAGRO/RS, Ministério da Agricultura, Pecuária e Abastecimento do Brasil, Estrada da Ponta Grossa 3036, Porto Alegre, RS, Brazil

    Rodrigo Hoff

Authors
  1. Márcia H. S. Kurz
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  2. Juliana L. da S. Batista
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  3. Lenise G. de Oliveira
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  4. Rodrigo Hoff
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  5. Manoel L. Martins
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  6. Fábio F. Gonçalves
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Correspondence to Márcia H. S. Kurz.

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Conflict of Interest

Márcia H. S. Kurz declares that she has no conflict of interest. Juliana L. da S. Batista declares that she has no conflict of interest. Lenise G. de Oliveira declares that she has no conflict of interest. Rodrigo Hoff declares that he has no conflict of interest. Manoel L. Martins declares that he has no conflict of interest. Fábio F. Gonçalves declares that he has no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Kurz, M.H.S., da S. Batista, J.L., de Oliveira, L.G. et al. Clean-up Procedure Development and Method Validation for Pesticide Residues Analysis in Carrots. Food Anal. Methods 12, 282–292 (2019). https://doi.org/10.1007/s12161-018-1359-y

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  • DOI: https://doi.org/10.1007/s12161-018-1359-y

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