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Quantification of Crustacean Tropomyosin, a Major Food Allergen, in Eight Species of Taiwanese Shrimp Based on Immunoassay

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Abstract

Shrimp is one of the most common food allergens in Taiwan. However, a simple method of measuring crustacean tropomyosin, the active allergen in shrimp is lacking. In this study, we aim to develop a validated enzyme-linked immunosorbent assay (ELISA) for quantifying level of tropomyosin in eight shrimp species in Taiwan. Our ELISA showed high specificity to shrimp tropomyosin, and inter-day precision and intra-day precision were 8.7% when 0.2 ng/ml were used as standard test samples. Furthermore, our ELISA had 0.030 ng/mL limited of detection (LOD) and 0.064 ng/mL limit of quantification (LOQ). We found despite the steaming process, levels of tropomyosin were still detectable which indicate active allergen in processed shrimps. Our findings in this study suggest this ELISA method can be a useful technique to study shrimp allergen for future crustacean allergen research.

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Abbreviations

ELISA:

enzyme-linked immunosorbent assay

PCR:

polymerase chain reaction

SDS-PAGE:

sodium dodecyl sulfate-polyacrylamide gel electrophoresis

WHO:

World Health Organization

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Funding

This research work was supported by grants from the Taiwan Ministry of Science and Technology (MOST 105-2320-B-020-001-) and Chang Gung Memorial Research Foundation (CORPG8F0023).

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Author notes

  1. Shin-You Lin and Chih-Hung Lee contributed equally to this work.

Authors and Affiliations

  1. Department of Food Science, National Pingtung University of Science and Technology, Pingtung, 912, Taiwan

    Shin-You Lin, Shyang-Chwen Sheu & Hsu-Sheng Yu

  2. Department of Dermatology, Kaohsiung Chang Gung Memorial Hospital and College of Medicine, Chang Gung University, Kaohsiung, 833, Taiwan

    Chih-Hung Lee

  3. Department of Gastroenterology, Palo Alto Medical Foundation, Mountain View, CA, 94040, USA

    Edward S. Huang

Authors
  1. Shin-You Lin
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  2. Chih-Hung Lee
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  3. Edward S. Huang
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  4. Shyang-Chwen Sheu
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  5. Hsu-Sheng Yu
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Corresponding author

Correspondence to Hsu-Sheng Yu.

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Shin-You Lin declares that he has no conflict of interest. Chih-Hung Lee declares that he has no conflict of interest. Edward S. Huang declares that he has no conflict of interest. Shyang-Chwen Sheu declares that she has no conflict of interest. Hsu-Sheng Yu declares that he has no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Lin, SY., Lee, CH., Huang, E.S. et al. Quantification of Crustacean Tropomyosin, a Major Food Allergen, in Eight Species of Taiwanese Shrimp Based on Immunoassay. Food Anal. Methods 11, 2607–2613 (2018). https://doi.org/10.1007/s12161-018-1242-x

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  • DOI: https://doi.org/10.1007/s12161-018-1242-x

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