Abstract
A fast ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) method was developed for quantitative determination of pesticide residues in pear. A fast modified acetate QuEChERS method without clean-up was used for sample preparation. Validation was performed according to SANTE guideline. Matrix effect results were significative for most part of compounds and thus a matrix-matched calibration was employed. The linear range of the method was from 2.5 to 100 μg kg−1. Recoveries were between 70 and 120% with precision ≤ 20%. Limit of quantification was 2.5 μg kg−1 for most compounds. Uncertainty results ranged from 22 to 49%. In real sample analyses, 21 compounds were quantified in concentrations between 3.3 and 1427 μg kg−1. Method proved to be simple, robust and effective to be applied in routine analysis.
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The authors acknowledge the financial support and fellowship granted by the Brazilian agencies CNPq, CAPES, and FINEP.
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This study was funded by the National Council of Scientific and Technological Development (CNPq), Brazil.
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Magali Kemmerich declares that he has no conflict of interest. Gabrieli Bernardi declares that he has no conflict of interest. Osmar D. Prestes declares that he has no conflict of interest. Martha B. Adaime declares that he has no conflict of interest. Renato Zanella declares that he has no conflict of interest.
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Kemmerich, M., Bernardi, G., Prestes, O.D. et al. Comprehensive Method Validation for the Determination of 170 Pesticide Residues in Pear Employing Modified QuEChERS Without Clean-Up and Ultra-High Performance Liquid Chromatography Coupled to Tandem Mass Spectrometry. Food Anal. Methods 11, 556–577 (2018). https://doi.org/10.1007/s12161-017-1026-8
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DOI: https://doi.org/10.1007/s12161-017-1026-8