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Evaluation and Optimization of Three Different Immunoassays for Rapid Detection Zearalenone in Fodders

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Abstract

Zearalenone (ZEN) is one of the most common contaminants in fodder with obvious reproduction toxicity and potential carcinogenicity to animals and humans. Thus, simple and sensitive methods are required for the detection of ZEN. In this work, the anti-ZEN monoclonal antibody (mAb) was prepared by hybridoma technique. Then, the mAb-based indirect competitive enzyme-linked immunosorbent assay (ic-ELISA), fluorescence polarization immunoassay (FPIA), and immunochromatographic assay (ICA) were evaluated and optimized for ZEN detection in spiked fodder samples. The ic-ELISA and FPIA showed recovery ranges from 80 to 100 %. The limit of detection (LOD) in ic-ELISA, FPIA and ICA were 0.06, 0.54, and 10 ng/mL, respectively. The detection ranges of IC20~IC80 were 2.89 to 115.36 ng/mL in ic-ELISA and 3.0 to 1052 ng/mL in FPIA. Amongst three immunoassays, the ic-ELISA was the most sensitive and stable, nevertheless, most time-consuming with narrower detection range. The FPIA showed good sensitivity, precision, and wide detection range, but it required specific tracer. ICA was a time-saving handy method but exhibited relatively lower precision and quantification compared to other two methods.

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References

  • Beloglazova NV, Eremin SA (2015) Rapid screening of aflatoxin B1 in beer by fluorescence polarization immunoassay. Talanta 142:170–175. doi:10.1016/j.talanta.2015.04.027

    Article  CAS  Google Scholar 

  • Beloglazova NV, Shmelin PS, Eremin SA (2016) Sensitive immunochemical approaches for quantitative (FPIA) and qualitative (lateral flow tests) determination of gentamicin in milk. Talanta 149:217–224. doi:10.1016/j.talanta.2015.11.060

    Article  CAS  Google Scholar 

  • Berthiller F et al. (2013) Masked mycotoxins: a review Molecular nutrition & food research 57:165–186

  • Choi EH, Kim DM, Choi SW, Eremin SA, HS C (2011) Optimisation and validation of a fluorescence polarisation immunoassay for rapid detection of zearalenone in corn. Int J Food Sci Technol 46:2173–2181

    Article  CAS  Google Scholar 

  • Chun HS, Choi EH, Chang HJ, Choi SW, SA E (2009) A fluorescence polarization immunoassay for the detection of zearalenone in corn. Anal Chim Acta 639:83–89. doi:10.1016/j.aca.2009.02.048

    Article  CAS  Google Scholar 

  • Commission Regulation (EC) No. 1881/2006 of 19 December, (2006). Setting maximum levels for certain contaminants in foodstuffs. Off J Eur Union. 364:5–24

  • Duan H, Chen X, Xu W, Fu J, Xiong Y, Wang A (2015) Quantum-DoT submicrobead-based immunochromatographic assay for quantitative and sensitive detection of zearalenone. Talanta 132:126–131

    Article  CAS  Google Scholar 

  • Gao Y, Yang M, Peng C, Li X, Cai R, Qi Y (2012) Preparation of highly specific anti-zearalenone antibodies by using the cationic protein conjugate and development of an indirect competitive enzyme-linked immunosorbent assay. Analyst 137:229–236. doi:10.1039/c1an15487g

    Article  CAS  Google Scholar 

  • Hadiani MR, Yazdanpanah H, MM G-K, Cheraghali A, Goodarzi M (2003) Survey of the natural occurrence of zearalenone in maize from northern Iran by thin-layer chromatography densitometry. Food Addit Contam 20:380–385

    Article  CAS  Google Scholar 

  • Huang PN, Zhao SQ, Eremin SA, Zheng SW, Lai D, Chen YS, Guo B (2015) A fluorescence polarization immunoassay method for detection of the bisphenol A residue in environmental water samples based on a monoclonal antibody and 4 ‘-(aminomethyl)fluorescein. Anal Methods-Uk 7:4246–4251. doi:10.1039/c5ay00818b

    Article  CAS  Google Scholar 

  • Huang Y, Xu Y, He Q, Chu J, B D, Liu J (2014) Determination of zearalenone in corn based on a biotin-avidin amplified enzyme-linked immunosorbent assay. Food Agric Immunol 25:186–199

    Article  CAS  Google Scholar 

  • Li X et al. (2013) Multi-component immunochromatographic assay for simultaneous detection of aflatoxin B 1, ochratoxin A and zearalenone in agro-food. Biosens Bioelectron 49:426–432

    Article  CAS  Google Scholar 

  • Lippolis V, Pascale M, Valenzano S, Porricelli ACR, Suman M, Visconti A (2014) Fluorescence polarization immunoassay for rapid, accurate and sensitive determination of ochratoxin A in wheat. Food Anal Methods 7:298–307

    Article  Google Scholar 

  • Mi T, Wang Z, Eremin SA, Shen J, Zhang S (2013) Simultaneous determination of multiple (fluoro) quinolone antibiotics in food samples by a one-step fluorescence polarization immunoassay. J Agric Food Chem 61:9347–9355

    Article  CAS  Google Scholar 

  • Mirocha C, Christensen C, Nelson G (2013) F-2 (zearalenone) estrogenic mycotoxin from fusarium. Microbial Toxins 7:107–138

    Google Scholar 

  • Streit E et al. (2012) Current situation of mycotoxin contamination and co-occurrence in animal feed—focus on Europe. Toxicology 4:788–809

    CAS  Google Scholar 

  • Sun Y et al. (2014) Development of an immunochromatographic strip test for the rapid detection of zearalenone in corn. J Agric Food Chem 62:11116–11121. doi:10.1021/jf503092j

    Article  CAS  Google Scholar 

  • Thyrhaug E, Sørensen TJ, Gryczynski I, Gryczynski Z, Laursen BW (2013) Polarization and symmetry of electronic transitions in long fluorescence lifetime triangulenium dyes. J Phys ChemA 117:2160–2168

    Article  CAS  Google Scholar 

  • Tian J, Zhou L, Zhao Y, Wang Y, Peng Y, Hong X, Zhao S (2012) The application of CdTe/CdS in the detection of carcinoembryonic antigen by fluorescence polarization immunoassay. J Fluoresc 22:1571–1579. doi:10.1007/s10895-012-1097-2

    Article  CAS  Google Scholar 

  • Tian X et al. (2015) Quantification of diethyl phthalate by a rapid and homogenous fluorescence polarization immunoassay. Anal Lett 48:2843–2855

    Article  CAS  Google Scholar 

  • Udenfriend S (2014) Fluorescence assay in biology and medicine vol 2. Academic Press.

  • Wang Y-K et al. (2013) Development of a rapid and simultaneous immunochromatographic assay for the determination of zearalenone and fumonisin B1 in corn, wheat and feedstuff samples. Food Control 31:180–188

    Article  CAS  Google Scholar 

  • Wang Y-K, Wang Y-C, Wang H-A, Ji W-H, Sun J-H, Yan Y-X (2014) An immunomagnetic-bead-based enzyme-linked immunosorbent assay for sensitive quantification of fumonisin B1. Food Control 40:41–45

    Article  Google Scholar 

  • Yang J-Y et al. (2015) Development of fluorescence polarisation immunoassay for carbofuran in food and environmental water samples. Food Agric Immunol 26:340–355

    Article  CAS  Google Scholar 

  • Yazdanpanah H, Zarghi A, Shafaati AR, Foroutan SM, Aboul-Fathi F, Khoddam A, Nazari F (2012) Exposure assessment of the Tehran population (Iran) to zearalenone mycotoxin. Iran J Pharma Res: IJPR 11:251

    CAS  Google Scholar 

  • Zhang W, Ye Z, Jin Y, Wang S, Zhang L, Pei X (2013) Management of mycotoxin contamination in food and feed in China. World Mycotoxin J 7:53–62

    Article  Google Scholar 

  • Zhu L et al. (2012) Zearalenone induces apoptosis and necrosis in porcine granulosa cells via a caspase-3-and caspase-9-dependent mitochondrial signaling pathway. J Cell Physiol 227:1814–1820

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This study was supported by the key construction program (S2015YFKW0002) of international cooperation base in S&T, Shaanxi Province, the major science and technology projects of Luoyang (150101A), Henan Engineering Laboratory for animal disease diagnosis and food safety detection (2015382), the Ph.D. Programs Foundation of Ministry of Education of China (20130204110023), SCO Regional collaborative innovation project (2016E03012), Xinjiang,International exchanges and cooperation project of Ministry of Agriculture, China, the research was supported by Grant of Russian Foundation for Basic Research (15-53-46015), Russian.

The authors thank Dr. Diraviyam Thirumalai for the language improvement.

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Authors and Affiliations

  1. College of Veterinary Medicine, Northwest A&F University, Post Box 19, Xinong Rd. 22, Yangling, Shaanxi Province, 712100, China

    Xuemei Jiang & Xiaoying Zhang

  2. Laipson Health Information Technology Co., Ltd, He’nan, Luoyang, 471000, China

    Xuemei Jiang, Xiumei Li & Zhi Yang

  3. Department of Chemical Enzymology, Faculty of Chemistry, M.V. Lomonosov State University, 119992, Moscow, Russia

    Sergei A. Eremin

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  1. Xuemei Jiang
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  2. Xiumei Li
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  4. Sergei A. Eremin
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Correspondence to Xiaoying Zhang.

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All procedures performed in studies involving animals were in accordance with the ethical standards of the Ethics Committee of Northwest A&F University for the use of Laboratory Animals.

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Xuemei Jiang declares that she has no conflict of interest. Xiumei Li declares that she has no conflict of interest. Zhi Yang declares that he has no conflict of interest. Sergei A. Eremin declares that he has no conflict of interest. Xiaoying Zhang declares that she has no conflict of interest.

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Jiang, X., Li, X., Yang, Z. et al. Evaluation and Optimization of Three Different Immunoassays for Rapid Detection Zearalenone in Fodders. Food Anal. Methods 10, 256–262 (2017). https://doi.org/10.1007/s12161-016-0576-5

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  • DOI: https://doi.org/10.1007/s12161-016-0576-5

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