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Simultaneous Determination of Phoxim, Chlorpyrifos, and Pyridaben Residues in Edible Mushrooms by High-Performance Liquid Chromatography Coupled to Tandem Mass Spectrometry

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Abstract

A new method for simultaneous determination of phoxim, chlorpyrifos, and pyridaben residues in edible mushrooms and the substrate at trace levels was developed using QuEChERS (quick, easy, cheap, efficient, rugged, and safe) analytical procedure and ultra-high-performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS). The target compounds were determinated using UHPLC-MS/MS with an electrospray ionization source in positive mode (ESI+). The limit of detection (LOD) for the three compounds ranged from 1 to 10 μg kg−1, and the limit of quantification (LOQ) was 10 μg kg−1. Quantification was performed by calibration curves of standards from 0.01 to 1.0 mg kg−1 with correlation coefficients >0.994. Recovery studies were performed at three spiked levels of 0.01, 0.05, and 0.25 mg kg−1 in blank mushrooms and the substrate, and the overall average recoveries ranged from 75.5 to 98.4 %. The data demonstrated the good repeatability of the method with relative standard deviations (RSDs) lower than 11.4 % for all analytes. The results showed that the method is efficient and reliable for the simultaneous determination of phoxim, chlorpyrifos, and pyridaben residues in mushrooms and substrate.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (31371970 and 31272070).

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Authors and Affiliations

  1. Institute of Plant Protection, Chinese Academy of Agricultural Sciences, State Key Laboratory for Biology of Plant Diseases and Insect Pests, Beijing, 100193, China

    Fajun Tian, Xingang Liu, Jun Xu, Fengshou Dong, Yongquan Zheng, Mingfeng Hu & Yanbing Wu

  2. Henan Institute of Science and Technology, Xinxiang, 453003, China

    Fajun Tian & Yanbing Wu

Authors
  1. Fajun Tian
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  2. Xingang Liu
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  3. Jun Xu
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  4. Fengshou Dong
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  5. Yongquan Zheng
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  6. Mingfeng Hu
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  7. Yanbing Wu
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Correspondence to Xingang Liu or Yanbing Wu.

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Tian, F., Liu, X., Xu, J. et al. Simultaneous Determination of Phoxim, Chlorpyrifos, and Pyridaben Residues in Edible Mushrooms by High-Performance Liquid Chromatography Coupled to Tandem Mass Spectrometry. Food Anal. Methods 9, 2917–2924 (2016). https://doi.org/10.1007/s12161-016-0490-x

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  • DOI: https://doi.org/10.1007/s12161-016-0490-x

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