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Rapid Determination of Antioxidant Compounds and Antioxidant Activity of Sudanese Karkade (Hibiscus sabdariffa L.) Using Near Infrared Spectroscopy

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Abstract

The extraction conditions applied in destructive analysis of Hibiscus sabdariffa L. (Hs) matrices before the measurement of antioxidant compounds may reduce their activities during isolation and purification due to decomposition. Hence, near infrared spectroscopic system was applied to determine these compounds. The calibration models developed by using partial least squares regression (PLSR) with 48 Hs samples and 24 unknown samples were used to confirm the robustness of the developed model. The results of calibration models effectively allowed the determination of the concentrations of total phenol content (TPC), total flavonoid content (TFC), total antioxidant content (TAC), ferric reducing antioxidant power (FRAP), and 1,1-diphenyl-2-picrylhydrazyl (DPPH) with high correlation coefficients (0.85–0.91) and small standard error of cross-validation (RMSECV) ranged from 0.08 to 0.33. The prediction capacity (RPD) for TPC, TFC, TAC, FRAP, and DPPH ranged from 3.30 to 8.25, thus demonstrating that the near infrared spectroscopic (NIRS) equations developed were applicable to unknown samples. This study demonstrates that NIRS can be considered as a fast tool for the nondestructive determination of antioxidant compounds (phenolics and flavonoids) and antioxidant activity of the Hs.

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Acknowledgments

Authors acknowledged the National Science and Technology support program (2015BAD17B04), the Jiangsu Province Science Fund for Distinguished Young Scholars (BK20130010), and New Century Excellent Talents in University (NCET-11-0986).

Conflict of Interest

Haroon Elrasheid Tahir declares that he has no conflict of interest. Zou Xiaobo declares that he has no conflict of interest. Shi Jiyong declares that he has no conflict of interest. Abdalbasit A. Mariod declares that he has no conflict of interest. Tchabo Wiliam declares that he has no conflict of interest.

Compliance with Ethics Requirements

This article does not contain any studies with human or animal subjects.

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Authors and Affiliations

  1. School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Rd., 212013, Zhenjiang, Jiangsu, China

    Haroon Elrasheid Tahir, Zou Xiaobo, Shi Jiyong & Tchabo Wiliam

  2. Key Laboratory of Modern Agricultural Equipment and Technology, 301 Xuefu Rd., 212013, Zhenjiang, Jiangsu, China

    Zou Xiaobo

  3. Department of Food Science and Technology, College of Agricultural Studies, Sudan University of Science and Technology, Khartoum, North Sudan

    Abdalbasit A. Mariod

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  1. Haroon Elrasheid Tahir
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  2. Zou Xiaobo
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  5. Tchabo Wiliam
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Correspondence to Zou Xiaobo.

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Tahir, H.E., Xiaobo, Z., Jiyong, S. et al. Rapid Determination of Antioxidant Compounds and Antioxidant Activity of Sudanese Karkade (Hibiscus sabdariffa L.) Using Near Infrared Spectroscopy. Food Anal. Methods 9, 1228–1236 (2016). https://doi.org/10.1007/s12161-015-0299-z

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