Abstract
A sensitive class-specific monoclonal antibody against tetracyclines (TCs) was generated and used to develop an enzyme-linked immunosorbent assay (ELISA) and an immunochromatographic assay for TC, oxytetracycline (OTC), and chlortetracycline (CTC) detection in milk and honey samples. The dynamic range of detection for TC in ELISA was 0.26–2.00 μg L−1 with an IC50 of 0.72 μg L−1. The IC50 value of OTC and CTC was 3.2 and 6.4 μg L−1, respectively. The recovery of TC, OTC, and CTC in milk samples was 82–102, 91–105, and 90–101 %, respectively, and 88–101, 89–101, and 89–95 % in honey samples, respectively. In the immunochromatographic assay, the cutoff values for TC, OTC, and CTC were 15, 15, and 50 μg L−1 in milk, respectively, and 40, 40, and 40 μg L−1 in honey, respectively. The results revealed that ELISA and the immunochromatographic assay can be applied for the rapid and sensitive detection of TC, OTC, and CTC in milk and honey samples.
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Acknowledgments
This work is financially supported by the Key Programs from MOST (2012BAK08B01, 2012BAD29B04).
Authors’ Contributions
Hua Kuang and Chuanlai Xu had the original idea for the study and, with all co-authors, carried out the design. Chuanlai Xu was responsible for the recruitment and the follow-up of study participants. Yanni Chen, Dezhao Kong, Liqiang Liu, and Shanshan Song were responsible the data cleaning, and Yanni Chen and Dezhao Kong carried out the analyses. Yanni Chen and Dezhao Kong drafted the manuscript, which was revised by Chuanlai Xu. All authors read and approved the final manuscript.
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Funding
Shanshan Song and Hua Kuang declare that they got a grant from MOST (2012BAK08B01). Chuanlai Xu declares that he got a grant from MOST (2012BAD29B04).
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Yanni Chen declares that she has no conflict of interest. Dezhao Kong declares that he has no conflict of interest. Liqiang Liu declares that she has no conflict of interest.
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This article does not contain any studies with human subjects. All animal studies were carried out under the guidance of the animal welfare committee of Jiangnan University.
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Chen, Y., Kong, D., Liu, L. et al. Development of an ELISA and Immunochromatographic Assay for Tetracycline, Oxytetracycline, and Chlortetracycline Residues in Milk and Honey Based on the Class-Specific Monoclonal Antibody. Food Anal. Methods 9, 905–914 (2016). https://doi.org/10.1007/s12161-015-0262-z
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DOI: https://doi.org/10.1007/s12161-015-0262-z