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A Fast and Accurate UPLC Method for Analysis of Proteinogenic Amino Acids

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Abstract

A reliable and inexpensive method for the measurement of proteinogenic amino acids was developed. It allows the screening of large sample volumes that is important for current food and feed applications, but also for future biobased applications. The method is based on acid hydrolysis and automated pre-column derivatision in the injection needle of the autosampler using o-phthalaldehyde/ethanethiol reagent in combination with 9-fluorenylmethyl chloroformate. The calculated mean limit of detection and limit of quantification of pure amino acids were 2.3 and 4.6 μM, respectively. With this method, it was possible to accurately analyse the amino acid composition of bovine serum albumin, soy meal, Jatropha press cake, and cyanophycin. Mean reproducibility of all amino acids from bovine serum albumin was 6.8 %, with a mean recovery of 95 %. The sample run time is 16 min with a total cycle time from injection to injection of an acid hydrolyzed sample of 22 min, resulting in a greener method due to a reduction in solvent consumption.

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Acknowledgments

This project was supported by a grant (EOSLT02034) provided by SenterNovem (Utrecht, the Netherlands).

Conflict of Interest

Bas J. Meussen has no conflict of interest. Albert N.T. van Zeeland has no conflict of interest. Marieke E. Bruins has no conflict of interest. Johan P.M. Sanders has no conflict of interest. This article does not contain any studies with human or animal subjects.

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Authors and Affiliations

  1. Biobased Commodity Chemicals, Wageningen University, Bornse Weilanden 9, 6708 WG, Wageningen, The Netherlands

    Bas J. Meussen, Albert N. T. van Zeeland, Marieke E. Bruins & Johan P. M. Sanders

  2. Fungal Systems Biology, Laboratory of Systems and Synthetic Biology, Wageningen University, Dreijenplein 10, 6703 HB, Wageningen, The Netherlands

    Bas J. Meussen

  3. Department Biobased Products, Agrotechnology and Food Sciences Group, WageningenUniversity and Research Centre, Bornse Weilanden 9, 6708 WG, Wageningen, The Netherlands

    Albert N. T. van Zeeland

Authors
  1. Bas J. Meussen
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  2. Albert N. T. van Zeeland
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  3. Marieke E. Bruins
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  4. Johan P. M. Sanders
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Corresponding author

Correspondence to Albert N. T. van Zeeland.

Additional information

Bas J. Meussen Albert N. T. van Zeeland contributed equally to this work.

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Meussen, B.J., van Zeeland, A.N.T., Bruins, M.E. et al. A Fast and Accurate UPLC Method for Analysis of Proteinogenic Amino Acids. Food Anal. Methods 7, 1047–1055 (2014). https://doi.org/10.1007/s12161-013-9712-7

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  • DOI: https://doi.org/10.1007/s12161-013-9712-7

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