Abstract
Pyrolysis molecular-beam mass spectrometry (PyMBMS) was tested as a high-throughput method for relative abundances of guaiacyl and syringyl lignin in lignocellulosic cell-wall materials from stems of a population of maize intermated B73 × Mo17 (IBM) recombinant inbred lines. Variations of up to twofold across the population in phenylpropanoid abundance were observed. Several histochemical and quantitative biochemical assays were used to validate the mass spectrometric data for lignin, hydroxycinnamic acids, crystalline cellulose, non-cellulosic glucans, and xylans. We demonstrate PyMBMS to be a valid high-throughput screen suitable for analysis of lignin abundance in large populations of bioenergy grasses. Pentose from xylans and hexose from cellulosic and non-cellulosic glucans also varied substantially across the population, but abundances of diagnostic fragments for these monosaccharides were not well correlated with the abundance of cell-wall polysaccharides.
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Abbreviations
- PyMBMS:
-
Pyrolysis molecular-beam mass spectrometry
- IBM:
-
Intermated B73 × Mo17 recombinant inbred population
- GAX:
-
Glucuronoarabinoxylan
- G-lignin:
-
Guaiacyl-lignin
- H-lignin:
-
p-Hydroxyphenol-lignin
- RIL:
-
Recombinant inbred line
- QTL:
-
Quantitative trait locus (loci)
- S-lignin:
-
Syringyl-lignin
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Acknowledgments
We thank Steve Moose, University of Illinois, for providing access to the maize stover and helpful discussions. Multiyear sampling of the IBM population was supported by the U.S. Department of Energy Feedstock Genomics Program, Office of Biological and Environmental Research, Office of Science; screening of the IBM population by PyMBMS was supported by the BioEnergy Science Center a U.S. Department of Energy Bioenergy Research Center supported by the Office of Biological and Environmental Research in the DOE Office of Science Contract No. DE-AC36-08-GO28308, and by the National Science Foundation “Hy-Bi”, an Emerging Frontiers in Research and Innovation (EFRI) program, Award No. 0938033; histochemical and analytical validations of PyMBMS for lignin and carbohydrate abundance were supported by the Center for Direct Catalytic Conversion of Biomass to Biofuels (C3Bio), an Energy Frontier Research Center funded by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences, Award Number DE-SC0000997.
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Penning, B.W., Sykes, R.W., Babcock, N.C. et al. Validation of PyMBMS as a High-throughput Screen for Lignin Abundance in Lignocellulosic Biomass of Grasses. Bioenerg. Res. 7, 899–908 (2014). https://doi.org/10.1007/s12155-014-9410-3
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DOI: https://doi.org/10.1007/s12155-014-9410-3