Abstract
The aim of this study was to evaluate the biological profile of odontogenic epithelium by immunolabeling of epidermal growth factor receptor (EGFR), Ki-67 and survivin in keratocystic odontogenic tumors (KOT), dentigerous cysts (DC), and pericoronal follicles (PF). Immunohistochemical analysis was performed in 13 KOTs, 14 DCs and 9 PFs. Immunolabeling was analyzed in the basal and suprabasal layers of KOTs and DCs, and in the islands of odontogenic epithelium and/or reduced enamel epithelium of PFs. KOTs showed the highest proliferation rate among the three groups, mainly in suprabasal layers. EGFR immunolabeling was observed mainly in the cytoplasm in basal and suprabasal layers of KOTs and in the suprabasal layer of DCs. Immunolabeling in both membrane and cytoplasm was greater in PFs. In PFs, membrane-only staining was observed. Survivin immunolabeling showed a greater percentage of positive cells (scoring +++) in the suprabasal layer of KOTs. In DCs, both layers showed similar percentages of cells scoring +++; PFs showed the highest percentage of these cells. In KOTs, epithelial cells showed stimulus-independent neoplastic proliferative characteristics, suggesting the presence of a suprabasal proliferative compartment, maintained by inhibition of apoptosis. In DCs, the basal layer seemed to proliferate in response to stimulus. Although PFs showed low proliferative activity, the expression of EGFR indicates that some cells have a high capacity to respond to stimuli, which could probably explain the origin of odontogenic lesions.
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Curran AE, Damm DD, Drummond JF. Pathologically significant pericoronal lesions in adults: histopathologic evaluation. J Oral Maxillofac Surg. 2002;60:613–7.
Aldelsperger J, Campbell JH, Coates DB, et al. Early soft tissue pathosis associated with impacted third molars without pericoronal radiolucency. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2000;89:402–6.
Baumgart SC, Lauxen IS, Sant’Ana Filho M, et al. Epidermal growth factor receptor distribution in pericoronal follicles: relationship with the origin of odontogenic cysts and tumors. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2007;103:240–5.
Edmatsu M, Kumanoto H, Ooya K, et al. Apoptosis-related factors in the epithelial components of pericoronal follicles and dentigerous cysts associated with impacted third molars of the mandible. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2005;99:17–23.
Payeras MR, Sant’Ana Filho M, Lauxen IS, et al. Quantitative analysis of argyrophilic nucleolar organizer regions and epidermal growth factor receptor in ameloblastomas. J Oral Pathol Med. 2007;36(2):99–104.
Kramer IRH, Pindborg JJ, Shear M. Histological typing of odontogenic tumours. Berlin, Germany: Springer; 1992.
Philipsen HP. Keratocyst odontogenic tumour. In: Barnes L, Eveson JW, Reichart P, Sidransky D, editors. World Health Organization classification of tumours. Lyon, France: IARC Press; 2005.
Slootweg PJ. p53 protein and Ki-67 reactivity in epithelial odontogenic lesions: an immunohistochemical study. J Oral Pathol Med. 1995;24:393–7.
Li TJ, Browne RM, Prime SS, et al. p53 expression in odontogenic keratocyst epithelium. J Oral Pathol Med. 1996;25:249–55.
Stoelinga PJW. Etiology and pathogenesis of keratocyst: natural history and immunohistochemistry. Oral Maxillofac Surg Clin North Am. 2003;15:317–24.
Kichi E, Enokiya Y, Muramatsu T, et al. Cell proliferation, apoptosis and apoptosis-related factors in odontogenic keratocysts and in dentigerous cysts. J Oral Pathol Med. 2005;34:280–6.
Laimer K, Spizzo G, Gastl G, et al. High EGFR expression predicts poor prognosis in patients with squamous cell carcinoma of the oral cavity and oropharynx: a TMA-based immunohistochemical analysis. Oral Oncol. 2007;43(2):193–8.
Herbst RS. Review of epidermal growth factor receptor biology. Int J Radiat Oncol Biol Phys. 2004;59:21–6.
Lin SY, Makino K, Xia W, et al. Nuclear localization of EGF receptor and its potential new role as a transcription factor. Nat Cell Biol. 2001;3(9):802–8.
Sah NK, Khan Z, Khan GJ, et al. Structural, functional and therapeutic biology of survivin. Cancer Lett. 2006;244(2):164–71.
Duffy MJ, O’Donovan N, Brennan DJ, et al. Survivin: a promising tumor biomarker. Cancer Lett. 2007;249:49–60.
Brennan DJ, Rexhepaj E, O’Brien SL, et al. Altered cytoplasmic-to-nuclear ratio of survivin is a prognostic indicator in breast cancer. Clin Cancer Res. 2008;14(9):2681–9.
Kumamoto H, Ooya K. Expression of survivin and X chromosome-linked inhibitor of apoptosis protein in ameloblastomas. Virchows Arch. 2004;444:164–70.
Andric M, Dozic B, Popovic B, et al. Survivin expression in odontogenic keratocysts and correlation with cytomegalovirus infection. Oral Dis. 2010;16:156–9.
Saraçoğlu U, Kurt B, Günhan Ö, et al. MIB-1 expression in odontogenic epithelial rests, epithelium of health oral mucosa and epithelium of selected odontogenic cysts: an immunohistochemical study. Int J Oral Maxillofac Surg. 2005;34:432–5.
Lo Muzio L, Staibano S, Pannone G, et al. Expression of cell cycle and apoptosis-related proteins in sporadic odontogenic keratocysts and odontogenic keratocysts associated with the nevoid basal cell carcinoma syndrome. J Dent Res. 1999;78:1345–53.
De Paula AMB, Carvalhais JN, Domingues MG, et al. Cell proliferation markers in the odontogenic keratocyst: effect of inflammation. J Oral Pathol Med. 2000;29:477–82.
Kaplan I, Hirshberg A. The correlation between epithelial cell proliferation and inflammation in odontogenic keratocyst. Oral Oncol. 2004;40:985–91.
Damjanov I, Mildner B, Knowles BB. Immunohistochemical localization of the epidermal growth factor receptor in normal human tissues. Lab Invest. 1986;55:588–92.
Gulkesen KH, Kilicarslan B, Altunbas HA, et al. EGFR and p53 expression and proliferative activity in parathyroid adenomas: an immunohistochemical study. APMIS. 2001;109:870–4.
Lo Muzio L, Pannone G, Leonardi R, et al. Survivin, a potential early predictor of tumor progression in the oral mucosa. J Dent Res. 2003;82:923–8.
Lo Muzio L, Farina A, Rubini C, et al. Survivin as prognostic factor in squamous cell carcinoma of the oral cavity. Cancer Lett. 2005;225:27–33.
Tanaka C, Uzawa K, Shibahana T, et al. Expression of an inhibitor of apoptosis, Survivin, in oral carcinogenesis. J Dent Res. 2003;82:607–11.
Pizen J, Cör A, Gale N. Survivin expression is a negative marker in laryngeal squamous cell carcinoma and is associated with p53 accumulation. Histopathology. 2004;45:180–6.
Suzuki T, Kumanoto H, Kunimore K, et al. Immunohistochemical analysis of apoptosis-related factors in lining epithelium of radicular cysts. J Oral Pathol Med. 2005;34:46–52.
Thosaporn W, Iamaroon A, Pongsiriwets S, et al. A comparative study of epithelial cell proliferation between the odontogenic keratocysts, orthokeratinized odontogenic cyst and ameloblastoma. Oral Dis. 2004;10:22–6.
Oliveira MG, Lauxen IS, Chaves ACM, et al. Immunohistochemical analysis of the patterns of p53 and PCNA expression in odontogenic cystic lesions. Med Oral Patol Oral Cir Bucal. 2008;13(5):E275–80.
Gurgel CAS, Ramos EAG, Azevedo RA, et al. Expression of Ki-67, p53 and p63 proteins in keratocyst odontogenic tumours: an immunohistochemistry study. J Mol Histol. 2008;39:311–6.
Gadbail AR, Chaudhary M, Patil S, et al. Actual Proliferating Index and p53 protein expression as prognostic marker in odontogenic cysts. Oral Dis. 2009;15:490–8.
Piatelli A, Lezzi G, Fioroni M, et al. Ki-67 expression in dentigerous cysts, unicystic ameloblastoma and ameloblastomas arising from dental cysts. J Endod. 2002;28(2):55–8.
Fortugno P, Wall NR, Giodini A, et al. Survivin exists in immunochemically distinct subcellular pools and is involved in spindle microtubule function. J Cell Sci. 2002;1151:575–85.
De Maria S, Pannone G, Bufo P, et al. Survivin gene-expression and splicing isoforms in oral squamous cell carcinoma. J Cancer Res Clin Oncol. 2009;135(1):107–16.
Li F. Role of surviving and its splice variants in tumorigenesis. Br J Cancer. 2005;92(2):212–6.
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de Oliveira, M.G., da Silva Lauxen, I., Chaves, A.C.M. et al. Odontogenic Epithelium: Immunolabeling of Ki-67, EGFR and Survivin in Pericoronal Follicles, Dentigerous Cysts and Keratocystic Odontogenic Tumors. Head and Neck Pathol 5, 1–7 (2011). https://doi.org/10.1007/s12105-010-0216-0
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DOI: https://doi.org/10.1007/s12105-010-0216-0