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Epstein–Barr Virus and Human Herpes Virus-8 are not Associated with Juvenile Nasopharyngeal Angiofibroma

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Abstract

Background Nasopharyngeal angiofibroma (also known as juvenile nasopharyngeal angiofibroma) is a rare fibroblastic tumor with a vascular component that occurs in the nasopharynx and posterolateral nasal wall of adolescent boys. The etiology of nasopharyngeal angiofibroma remains elusive. This investigation was undertaken to determine if human herpes simplex virus-8 and Epstein–Barr virus are possible etiologic viruses and to determine if they have any association with the age of the patient and/or the proliferative state of the lesion. Materials and methods Formalin fixed, routinely processed, and paraffin embedded surgical specimens of 15 angiofibromas were submitted to PCR for EBV and HHV-8, while in situ hybridization was also employed for EBV. Immunohistochemical analysis for ki-67 was performed using MIB immunostaining. Results None of the tumors were positive for HHV-8. The PCR technique produced a false positive reaction in five cases, with all cases non-reactive with EBV-ISH. The age of the patients did not show correlation with the Ki-67 labeling index. Conclusion Angiofibroma does not appear to be associated with either HHV-8 or EBV, thereby excluding these viruses as potential etiologic agents. The lack of a correlation between the proliferative index and the age of the patient suggests the proposed puberty induced, testosterone-dependent tumor growth may not play a significant role in tumor development.

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Acknowledgements

Grant sponsor: Milênio CNPq/MCT and FAPEMIG, Brazil. Dr. RS Gomez is research fellow of CNPq, Brazil. The opinions or assertions contained herein are the private views of the authors and are not to be construed as official or as reflecting the views of the affiliated Institutions. There is no financial conflict of interest.

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Authors and Affiliations

  1. Departmento de Patologia, Centro Clinico de Cabeza e Cuello, Guatemala, Guatemala

    Román Carlos & Ana Carolina Netto

  2. Department of Pathology, Southern California Permanente Medical Group, Woodland Hills, CA, USA

    Lester D. R. Thompson

  3. Departments of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil

    Luiz Gustavo Garcia Santos Pimenta, Jeane de Fátima Correia-Silva, Carolina Cavaliéri Gomes & Ricardo Santiago Gomez

  4. Departamento de Patologia e Cirurgia, Faculdade de Odontologia, Universidade Federal de Minas Gerais, Av. Antonio Carlos, 6627, Belo Horizonte, MG, CEP 31270-901, Brazil

    Ricardo Santiago Gomez

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  1. Román Carlos
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  2. Lester D. R. Thompson
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  3. Ana Carolina Netto
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  4. Luiz Gustavo Garcia Santos Pimenta
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Correspondence to Ricardo Santiago Gomez.

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Carlos, R., Thompson, L.D.R., Netto, A.C. et al. Epstein–Barr Virus and Human Herpes Virus-8 are not Associated with Juvenile Nasopharyngeal Angiofibroma. Head and Neck Pathol 2, 145–149 (2008). https://doi.org/10.1007/s12105-008-0069-y

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  • DOI: https://doi.org/10.1007/s12105-008-0069-y

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