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Human Kallikrein 8 Expression in Salivary Gland Tumors

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Abstract

The human kallikrein 8 protein (KLK8) is expressed in many normal tissues including esophagus, skin, testis, tonsil, kidney, breast, and salivary gland, and is found in biological fluids including breast milk, amniotic fluid, seminal fluid and serum. It has also been shown to be a biomarker and prognostic factor for breast cancer. The aim of this study was to determine whether KLK8 is expressed in salivary gland tissues and salivary gland tumors (both benign and malignant), in order to compare normal with tumor tissues. Pleomorphic adenomas, adenoid cystic carcinomas, polymorphous low grade adenocarcinomas, acinic cell carcinomas, mucoepidermoid carcinomas, and adenocarcinomas NOS of both minor and major salivary glands were examined. The results of this study indicate that most salivary gland tumors show high levels of expression of KLK8.

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Abbreviations

PA:

Pleomorphic adenoma

ACC:

Adenoid cystic carcinoma

PLGA:

Polymorphous low grade adenocarcinoma

ACI:

Acinic cell carcinoma

MEC:

Mucoepidermoid carcinoma

ANOS:

Adenocarcinoma not otherwise specified

KLK:

Human kallikrein protein

KLK :

Human kallikrein gene

RT:

Room temperature

PBS:

Phosphate-buffered saline

Pab:

Polyclonal antibody

DAB:

3,3′-Diaminobenzidine

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Acknowledgments

The authors are grateful to Linda Grass for technical advice and to the National Organization for Rare Disorders which provided funding for this study.

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Authors and Affiliations

  1. Division of Oral Pathology, Department of Pathology, University of Western Ontario, 1151 Richmond Street, London, ON, Canada, N6A 5C1

    Mark R. Darling, Linda Jackson-Boeters & Thomas D. Daley

  2. Faculty of Medicine & Dentistry, University of Alberta, Edmonton, AB, Canada

    Sam Tsai

  3. Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, Toronto, ON, Canada

    Eleftherios P. Diamandis

  4. Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada

    Eleftherios P. Diamandis

Authors
  1. Mark R. Darling
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  2. Sam Tsai
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  3. Linda Jackson-Boeters
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  4. Thomas D. Daley
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  5. Eleftherios P. Diamandis
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Correspondence to Mark R. Darling.

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Darling, M.R., Tsai, S., Jackson-Boeters, L. et al. Human Kallikrein 8 Expression in Salivary Gland Tumors. Head and Neck Pathol 2, 169–174 (2008). https://doi.org/10.1007/s12105-008-0068-z

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  • DOI: https://doi.org/10.1007/s12105-008-0068-z

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