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NMR assignment of the amylase-binding protein A from Streptococcus parasanguinis

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Abstract

Streptococcus parasanguinis is a primary colonizer of tooth surfaces in the oral cavity. Amylase-binding protein A (AbpA) from S. parasanguinis is responsible for the recruitment of salivary amylase to bacterial surface, which plays an important role in the development of oral biofilms. Here, we describe the essentially complete NMR assignments for AbpA.

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Abbreviations

AbpA:

Amylase-binding protein A

IPTG:

Isopropyl-d-thiogalactopyranoside

NOE:

Nuclear overhauser effect

NOESY:

Nuclear overhauser effect enhancement spectroscopy

TOCSY:

Total correlation spectroscopy

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Acknowledgments

This work was supported by a Wellcome Trust Senior Investigator Award to SM and NIH/NIDCR DE022673 and DE017954.

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Correspondence to Stephen Matthews.

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Liu, B., Zhu, F., Wu, H. et al. NMR assignment of the amylase-binding protein A from Streptococcus parasanguinis . Biomol NMR Assign 9, 173–175 (2015). https://doi.org/10.1007/s12104-014-9568-9

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  • DOI: https://doi.org/10.1007/s12104-014-9568-9

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