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1H, 15N and 13C resonance assignments and secondary structure prediction of Q4D059, a conserved and kinetoplastid-specific hypothetical protein from Trypanosoma cruzi

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Abstract

Trypanosoma cruzi is a human parasite that causes Chagas disease, an illness affecting millions of people and without an efficient treatment available. Sequencing the pathogen genome has revealed that near half of protein-coding genes correspond to hypothetical proteins of unknown function, increasing the possibilities for novel target discovery. Q4D059 is a putative essential hypothetical protein from T. cruzi and it is specific and conserved among the trypanosomatid genomes. Here, we report the sequential backbone and side chain resonance assignments and secondary structure analysis of Q4D059, as first step for protein structure determination, function elucidation and drug screening.

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Acknowledgments

Financial support was obtained from CNPq, CAPES and FAPERJ (Brazilian funding agencies).

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Authors and Affiliations

  1. Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Av. Brigadeiro Trompowiski s/n CCS, Bloco E, sala 10, Rio de Janeiro, RJ, 21941-590, Brazil

    Aracelys López-Castilla, Rachel S. de Menezes, Éverton D. D’ Andrea, Thamires R. dos Santos & José R. Pires

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  1. Aracelys López-Castilla
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  2. Rachel S. de Menezes
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  3. Éverton D. D’ Andrea
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  4. Thamires R. dos Santos
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  5. José R. Pires
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Correspondence to José R. Pires.

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López-Castilla, A., de Menezes, R.S., D’ Andrea, É.D. et al. 1H, 15N and 13C resonance assignments and secondary structure prediction of Q4D059, a conserved and kinetoplastid-specific hypothetical protein from Trypanosoma cruzi . Biomol NMR Assign 9, 161–163 (2015). https://doi.org/10.1007/s12104-014-9565-z

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  • DOI: https://doi.org/10.1007/s12104-014-9565-z

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