Abstract
Acyl carrier proteins (ACPs) are a group of highly conserved and abundant proteins in bacteria. ACPs play a central role as the acyl group carriers in bacterial fatty acid biosynthesis, providing building blocks for membrane biogenesis and the production of secondary metabolites. In the versatile human pathogen Pseudomonas aeruginosa, three ACP homologs have been identified. One homolog, AcpP, exhibits the strongest sequence homology to the canonical Escherichia coli ACP. Here we report the 1H, 13C and 15N assignments of the holo-AcpP of P. aeruginosa.
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References
Breidenstein EBM, de la Fuente-Nunez C, Hancock REW (2011) Pseudomonas aeruginosa: all roads lead to resistance. Trends Microbiol 19(8):419–426
Chan DI, Vogel HJ (2010) Current understanding of fatty acid biosynthesis and the acyl carrier protein. Biochem J 430(1):1–19
Jacobs MA, Alwood A, Thaipisuttikul I, Spencer D, Haugen E, Ernst S, Will O, Kaul R, Raymond C, Levy R, Chun-Rong L, Guenthner D, Bovee D, Olson MV, Manoil C (2003) Comprehensive transposon mutant library of Pseudomonas aeruginosa. Proc Natl Acad Sci U S A 100(24):14339–14344
Li Z, Kosorok MR, Farrell PM, Laxova A, West SE, Green CG, Collins J, Rock MJ, Splaingard ML (2005) Longitudinal development of mucoid Pseudomonas aeruginosa infection and lung disease progression in children with cystic fibrosis. JAMA 293(5):581–588
Liberati NT, Urbach JM, Miyata S, Lee DG, Drenkard E, Wu G, Villanueva J, Wei T, Ausubel FM (2006) An ordered, nonredundant library of Pseudomonas aeruginosa strain PA14 transposon insertion mutants. Proc Natl Acad Sci U S A 103(8):2833–2838
Maier T, Leibundgut M, Boehringer D, Ban N (2010) Structure and function of eukaryotic fatty acid synthases. Q Rev Biophys 43(3):373–422
Neidhardt FC, Bloch PL, Smith DF (1974) Culture medium for enterobacteria. J Bacteriol 119(3):736–747
Raychaudhuri A, Jerga A, Tipton PA (2005) Chemical mechanism and substrate specificity of RhlI, an acylhomoserine lactone synthase from Pseudomonas aeruginosa. Biochemistry (Mosc) 44(8):2974–2981
Shen Y, Delaglio F, Cornilescu G, Bax A (2009) TALOS+: a hybrid method for predicting protein backbone torsion angles from NMR chemical shifts. J Biomol NMR 44(4):213–223
White SW, Zheng J, Zhang YM, Rock CO (2005) The structural biology of type II fatty acid biosynthesis. Annu Rev Biochem 74:791–831
Wishart DS, Arndt D, Berjanskii M, Tang P, Zhou J, Lin G (2008) CS23D: a web server for rapid protein structure generation using NMR chemical shifts and sequence data. Nucleic Acids Res 36(Web Server issue):W496–W502
Wu BN, Zhang YM, Rock CO, Zheng J (2009) Structural modification of acyl carrier protein by butyryl group. Protein Sci 18:240–246
Zhang YM, White SW, Rock CO (2006) Inhibiting bacterial fatty acid synthesis. J Biol Chem 281:17541–17544
Acknowledgments
This work was supported in part by NIH grants P20RR017677 (SC COBRE in Lipidomics & Pathobiology) and UL1RR029882 (SC Clinical & Translational Research Institute).
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Duggan, B.M., Roca, A. & Zhang, YM. 1H, 13C and 15N assignments of the holo-acyl carrier protein of Pseudomonas aeruginosa . Biomol NMR Assign 7, 225–228 (2013). https://doi.org/10.1007/s12104-012-9415-9
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DOI: https://doi.org/10.1007/s12104-012-9415-9