Skip to main content
SpringerLink
Log in

1H, 15N and 13C resonance assignment of imidazole glycerol phosphate (IGP) synthase protein HisF from Thermotoga maritima

  • Article
  • Published:
Biomolecular NMR Assignments Aims and scope Submit manuscript

Abstract

HisF comprises one half of the heterodimeric protein complex imidazole glycerol phosphate (IGP) synthase responsible for the fifth step of histidine biosynthesis. Here we report backbone and side chain assignments necessary for characterization of protein dynamics involved in the allosteric mechanism of IGP synthase.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Beismann-Driemeyer S, Sterner R (2001) Imidazole glycerol phosphate synthase from Thermotoga maritima—quaternary structure, steady-state kinetics, and reaction mechanism of the bienzyme complex. J Biol Chem 276(23):20387–20396. doi:10.1074/jbc.M102012200

    Article  Google Scholar 

  • Delaglio F, Grzesiek S et al (1995) NMRPipe—a multidimensional spectral processing system based on unix pipes. J Biomol NMR 6(3):277–293. doi:10.1007/BF00197809

    Article  Google Scholar 

  • Douangamath A, Walker M et al (2002) Structural evidence for ammonia tunneling across the (beta alpha) (8) barrel of the imidazole glycerol phosphate synthase bienzyme complex. Structure 10(2):185–193. doi:10.1016/S0969-2126(02)00702-5

    Article  Google Scholar 

  • Kneller DG, Kuntz ID (1993) Ucsf Sparky: an NMR display, annotation and assignment tool. J Cell Biochem 53:254

    Google Scholar 

  • Lang D, Thoma R et al (2000) Structural evidence for evolution of the beta/alpha barrel scaffold by gene duplication and fusion. Science 289(5484):1546–1550. doi:10.1126/science.289.5484.1546

    Article  ADS  Google Scholar 

  • Myers RS, Amaro RE et al (2005) Reaction coupling through interdomain contacts in imidazole glycerol phosphate synthase. Biochemistry 44(36):11974–11985. doi:10.1021/bi050706b

    Article  Google Scholar 

  • Myers RS, Jensen JR et al (2003) Substrate-induced changes in the ammonia channel for imidazole glycerol phosphate synthase. Biochemistry 42(23):7013–7022. doi:10.1021/bi034314l

    Article  Google Scholar 

  • Pervushin K, Riek R et al (1997) Attenuated T-2 relaxation by mutual cancellation of dipole-dipole coupling and chemical shift anisotropy indicates an avenue to NMR structures of very large biological macromolecules in solution. Proc Natl Acad Sci USA 94(23):12366–12371. doi:10.1073/pnas.94.23.12366

    Article  ADS  Google Scholar 

  • Smith DWE, Ames BN (1964) Intermediates in early steps of histidine biosynthesis. J Biol Chem 239(6):1848

    Google Scholar 

  • Thoma R, Obmolova G et al (1999) Efficient expression, purification and crystallisation of two hyperthermostable enzymes of histidine biosynthesis. FEBS Lett 454(1–2):1–6. doi:10.1016/S0014-5793(99)00757-7

    Article  Google Scholar 

  • Wishart DS, Sykes BD (1994) The 13C chemical-shift index: a simple method for the identification of protein secondary structure using 13C chemical-shift data. J Biomol NMR 4(2):171–180. doi:10.1007/BF00175245

    Article  Google Scholar 

Download references

Acknowledgments

This work was supported by a NIH grant (R01-GM070823) awarded to JPL. JML would like to thank fellowship support from a NIH biophysical training grant (5T32GM008283).

Author information

Authors and Affiliations

  1. Department of Chemistry, Yale University, Sterling Chemistry Laboratory, New Haven, CT, 06511, USA

    James M. Lipchock & J. Patrick Loria

  2. Department of Chemistry, Yale University, 225 Prospect Street, 208107, New Haven, CT, 06511, USA

    J. Patrick Loria

Authors
  1. James M. Lipchock
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Patrick Loria
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to J. Patrick Loria.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lipchock, J.M., Patrick Loria, J. 1H, 15N and 13C resonance assignment of imidazole glycerol phosphate (IGP) synthase protein HisF from Thermotoga maritima . Biomol NMR Assign 2, 219–221 (2008). https://doi.org/10.1007/s12104-008-9125-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12104-008-9125-5

Keywords

Search

Navigation