Skip to main content
Log in

1H, 13C, and 15N resonance assignments of a general stress protein GSP13 from Bacillus subtilis

  • Article
  • Published:
Biomolecular NMR Assignments Aims and scope Submit manuscript

Abstract

GSP13 encoded by gene yugI is a general stress protein in Bacillus subtilis. The NMR assignments of the protein are essential for its structure determination.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Antelmann H, Bernhardt J, Schmid R, Mach H, Volker U, Hecker M (1997) First steps from a two-dimensional protein index towards a response-regulation map for Bacillus subtilis. Electrophoresis 18:1451–1463. doi:10.1002/elps.1150180820

    Article  Google Scholar 

  • Bernhardt J, Volker U, Volker A, Antelmann H, Schmid R, Mach H et al (1997) Specific and general stress proteins in Bacillus subtilis—a two-dimensional protein electrophoresis study. Microbiology 143:999–1017

    Article  Google Scholar 

  • Bycroft M, Hubbard TJ, Proctor M, Freund SM, Murzin AG (1997) The solution structure of the S1 RNA binding domain: a member of an ancient nucleic acid-binding fold. Cell 88:235–242. doi:10.1016/S0092-8674(00)81844-9

    Article  Google Scholar 

  • Delaglio F, Grzesiek S, Vuister GW, Zhu G, Pfeifer J, Bax A (1995) NMRPipe—a multidimensional spectral processing system based on unix pipes. J Biomol NMR 6:277–293. doi:10.1007/BF00197809

    Article  Google Scholar 

  • Haldenwang WG, Losick R (1980) Novel RNA polymerase sigma factor from Bacillus subtilis. Proc Natl Acad Sci USA 77:7000–7004. doi:10.1073/pnas.77.12.7000

    Article  ADS  Google Scholar 

  • Hecker M, Pane-Farre J, Volker U (2007) SigB-dependent general stress response in Bacillus subtilis and related gram-positive bacteria. Annu Rev Microbiol 61:215–236. doi:10.1146/annurev.micro.61.080706.093445

    Article  Google Scholar 

  • Helmann JD, Wu MF, Kobel PA, Gamo FJ, Wilson M, Morshedi MM et al (2001) Global transcriptional response of Bacillus subtilis to heat shock. J Bacteriol 183:7318–7328. doi:10.1128/JB.183.24.7318-7328.2001

    Article  Google Scholar 

  • Johnson BA, Blevins RA (1994) NMRView: a computer program for the visualization and analysis of NMR data. J Biomol NMR 4:603–614. doi:10.1007/BF00404272

    Article  Google Scholar 

  • Kaan T, Homuth G, Mader U, Bandow J, Schweder T (2002) Genome-wide transcriptional profiling of the Bacillus subtilis cold-shock response. Microbiology 148:3441–3455

    Google Scholar 

  • Markley JL, Bax A, Arata Y, Hilbers CW, Kaptein R, Sykes BD et al (1998) Recommendations for the presentation of NMR structures of proteins and nucleic acids—IUPAC-IUBMB-IUPAB inter-union task group on the standardization of data bases of protein and nucleic acid structures determined by NMR spectroscopy. J Biomol NMR 12:1–23. doi:10.1023/A:1008290618449

    Article  Google Scholar 

  • Petersohn A, Brigulla M, Haas S, Hoheisel JD, Volker U, Hecker M (2001) Global analysis of the general stress response of Bacillus subtilis. J Bacteriol 183:5617–5631. doi:10.1128/JB.183.19.5617-5631.2001

    Article  Google Scholar 

  • Price CW, Fawcett P, Ceremonie H, Su N, Murphy CK, Youngman P (2001) Genome-wide analysis of the general stress response in Bacillus subtilis. Mol Microbiol 41:757–774. doi:10.1046/j.1365-2958.2001.02534.x

    Article  Google Scholar 

  • Wishart DS, Sykes BD (1994) The 13C chemical-shift index: a simple method for the identification of protein secondary structure using 13C chemical-shift data. J Biomol NMR 4:171–180. doi:10.1007/BF00175245

    Article  Google Scholar 

Download references

Acknowledgements

All NMR experiments were carried out at the Beijing NMR Center. We thank Dr. Xiaodong Su for providing us the genome library of Bacillus subtilis. This research was supported by Grant 30125009 to BX from the National Science Foundation of China and grant 2006AA02A323 to CJ from the 863 Program.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Bin Xia.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yu, W., Yu, B., Hu, J. et al. 1H, 13C, and 15N resonance assignments of a general stress protein GSP13 from Bacillus subtilis . Biomol NMR Assign 2, 163–165 (2008). https://doi.org/10.1007/s12104-008-9111-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12104-008-9111-y

Keywords

Navigation