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NMR assignment of the apo-form of a Desulfovibrio gigas protein containing a novel Mo–Cu cluster

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Abstract

We report the 98% assignment of the apo-form of an orange protein, containing a novel Mo–Cu cluster isolated from Desulfovibrio gigas. This protein presents a region where backbone amide protons exchange fast with bulk solvent becoming undetectable. These residues were assigned using 13C-detection experiments.

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Acknowledgements

This work was supported by a research project from the Fundação para a Ciência e Tecnologia (POCI/QUI/55350/2004). SRP, AGD and MSC would like to thank the Fundação para a Ciência e Tecnologia for financial support (POCI/SFRH/BPD/14067/2003, POCI/QUI/55350/2004, POCI/SFRH/BPD/24150/2005, respectively). NMR experiments were run at the CERM EU-NMR Research Infrastructure (Contract n° 026145), and SRP would like to thank the EU-NMR for the financial support during her visits to CERM.

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Authors and Affiliations

  1. REQUIMTE/CQFB, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica, 2829-516, Portugal

    Sofia R. Pauleta, Américo G. Duarte, Marta S. Carepo, Alice S. Pereira, Pedro Tavares, Isabel Moura & José J. G. Moura

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  1. Sofia R. Pauleta
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  2. Américo G. Duarte
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  3. Marta S. Carepo
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  4. Alice S. Pereira
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  5. Pedro Tavares
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  6. Isabel Moura
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  7. José J. G. Moura
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Correspondence to Sofia R. Pauleta.

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Pauleta, S.R., Duarte, A.G., Carepo, M.S. et al. NMR assignment of the apo-form of a Desulfovibrio gigas protein containing a novel Mo–Cu cluster. Biomol NMR Assign 1, 81–83 (2007). https://doi.org/10.1007/s12104-007-9022-3

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  • DOI: https://doi.org/10.1007/s12104-007-9022-3

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