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Dynamic Distribution and Clinical Value of Peripheral Lymphocyte Subsets in Children with Infectious Mononucleosis

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Abstract

Objective

To study the dynamic change of peripheral lymphocyte subsets and its clinical value in children with infectious mononucleosis (IM).

Methods

Thirty-six pediatric patients with IM, 19 children with IM-like symptoms but lacking the serological pattern compatible with EB virus infection, and 33 healthy children were enrolled. The changes of peripheral lymphocyte subsets were detected by flow cytometry on admission and on the fifth day of antiviral treatment, respectively. Indicators of liver function and routine blood count were also detected. Besides, the receiver operating characteristic (ROC) curve and the correlation of related indicators was analyzed.

Results

When IM patients were admitted, the frequency and absolute number of T, CD4−CD8+T, and CD4+CD8+T (DPT) cells were significantly increased while B cells were decreased; the frequency of CD4+CD8−T cells were decreased, but its absolute number did not change significantly; the frequency of NK cells decreased, but its absolute number increased. The absolute number of CD4−CD8+T most significantly positively correlated with serum lactate dehydrogenase (LDH) concentration which could reflect the severity of IM patients. After short-term treatment with acyclovir, elevated lymphocytes decreased, but only DPT-cell frequency and NK-cell absolute number were recovering towards normal. The ROC curve suggested that the frequency of B cells has better diagnostic value for IM in pediatric patients compared to other lymphocyte subsets.

Conclusions

Peripheral lymphocyte subsets are closely related to the condition of children with IM, and each subset plays a relatively different role in the diagnosis and evaluation of IM.

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Acknowledgements

The authors thank all children and their parents who agreed for the participation in this study.

Author information

Author notes

  1. The authors Liling Chen and Xinyuan Chen should be considered co-first authors and contributed equally to this work.

Authors and Affiliations

  1. School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, China

    Liling Chen & Xiaoyuan Liu

  2. Department of Clinical Laboratory, Wenzhou Central Hospital, Wenzhou, China

    Xinyuan Chen

  3. Department of Clinical Laboratory, The First Affiliated Hospital of Wenzhou Medical University, Nanbaixiang Street, OuHai District, Wenzhou, 325000, People’s Republic of China

    Weifeng Yao, Yujie Jiang, Jingjing Guan, Yaosheng Xie, Hong Lu, Jingjing Qian, Zhuo Zhang, Lianfeng Wu & Xiangyang Lin

  4. Department of Clinical Laboratory, The First Affiliated Hospital of Anhui Medical University, Hefei, China

    Xin Wei

Authors
  1. Liling Chen
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  2. Xinyuan Chen
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  4. Xin Wei
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Contributions

LLC and XYC: Principal investigators, conducted the study and collected the data; WFY and XW: Helped in data collection and participant interviewing; YJJ, JJG, and XYL: Supervised clinical data collection and clinical aspects of the study; YSX, HL, JJQ, ZZ, and LFW: Biostatistical analysis. XYL is the guarantor for this paper.

Corresponding author

Correspondence to Xiangyang Lin.

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Conflict of Interest

None.

Source of Funding

This study was supported by the Natural Science Foundation of Zhejiang Province of China (LY17H200005).

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Chen, L., Chen, X., Yao, W. et al. Dynamic Distribution and Clinical Value of Peripheral Lymphocyte Subsets in Children with Infectious Mononucleosis. Indian J Pediatr 88, 113–119 (2021). https://doi.org/10.1007/s12098-020-03319-7

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  • DOI: https://doi.org/10.1007/s12098-020-03319-7

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