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Application of Flow Cytometry in the Evaluation of Primary Immunodeficiencies

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Abstract

Primary immunodeficiency disorders (PIDDs) are a heterogeneous group of inherited disorders of the immune system. Currently more than 250 different PIDDs with a known genetic defect have been recognized. The diagnosis of many of these disorders is supported strongly by a wide variety of flow cytometry applications. Flow cytometry offers a rapid and sensitive tool for diagnosis and classification of PIDDs. It is applicable in the initial workup and subsequent management of several primary immunodeficiency diseases. As our understanding of the pathogenesis and management of these diseases increases, the majority of these tests can be easily established in the diagnostic laboratory. Thus, the focus of this article is on the application of flow cytometry in the diagnosis and/or evaluation of PIDDs.

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Contributions

TF, MM and SR contributed in reviewing the articles and writing the paper. TF will act as guarantor for the paper.

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Authors and Affiliations

  1. Department of Laboratory Medicine, NIH Clinical Center, Building 10, Room 2C306, 10 Center Drive, MSC1508, Bethesda, MD, USA

    Thomas A. Fleisher & Sergio D. Rosenzweig

  2. Department of Pediatric Immunology and Leukocyte Biology, National Institute of Immunohaematology (ICMR), KEM Campus, Parel, Mumbai, India

    Manisha Madkaikar

Authors
  1. Thomas A. Fleisher
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  2. Manisha Madkaikar
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  3. Sergio D. Rosenzweig
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Correspondence to Thomas A. Fleisher.

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Source of Funding

This work was supported by the Intramural Research Program of the NIH Clinical Center, National Institutes of Health, Bethesda, MD, USA.

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Fleisher, T.A., Madkaikar, M. & Rosenzweig, S.D. Application of Flow Cytometry in the Evaluation of Primary Immunodeficiencies. Indian J Pediatr 83, 444–449 (2016). https://doi.org/10.1007/s12098-015-2011-0

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  • DOI: https://doi.org/10.1007/s12098-015-2011-0

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