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Involvement of RalB in the effect of geranylgeranyltransferase I on glioma cell migration and invasion

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Abstract

Purpose

The present study aimed at investigating the role and potential mechanism of geranylgeranyltransferase I (GGTase-I) on glioma cell migration and invasion.

Methods

Wound-healing assay and transwell assay were performed to study whether GGTase-I and Ras-like GTPase B (RalB) have effect on glioma cell migration and invasion.

Results

We found that knockdown of GGTase-I or RalB both significantly decreased the migratory and invasive abilities of glioma cells. GGTase-I down-regulation suppressed RalB membrane association. Moreover, down-regulation of RalB partially abolished the effect of GGTβ over-expression-induced glioma cell migration and invasion increase.

Conclusions

These findings suggest that RalB might be one of the targets for facilitating the invasive phenotype of malignant gliomas induced by GGTase-I.

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Acknowledgments

The project was supported by the National Natural Science Foundation of China (No. 81272777; No. 81372699; No. 81302175; No. 81472345) and Natural Science Foundation of the Jiangsu Higher Education Institutions of China (No. 13KJB320025).

Conflict of interest

The authors declare no conflict of interest.

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Authors and Affiliations

  1. Institute of Nervous System Diseases, Xuzhou Medical College, 84 West Huai-hai Road, Xuzhou, 221002, Jiangsu, People’s Republic of China

    X. Song, L. Hua, Q. Shi, X. Zhou & R. Yu

  2. Brain Hospital, Affiliated Hospital of Xuzhou Medical College, 99 West Huai-hai Road, Xuzhou, 221002, Jiangsu, People’s Republic of China

    X. Song, L. Hua, Q. Shi, X. Zhou & R. Yu

  3. The Graduate School, Xuzhou Medical College, Xuzhou, Jiangsu, People’s Republic of China

    Y. Xu, Z. Fang, Y. Wang & J. Gao

Authors
  1. X. Song
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  2. L. Hua
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  3. Y. Xu
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  4. Z. Fang
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  5. Y. Wang
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  6. J. Gao
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  7. Q. Shi
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  8. X. Zhou
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  9. R. Yu
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Corresponding authors

Correspondence to X. Zhou or R. Yu.

Additional information

X. Song, L. Hua and Y. Xu have contributed equally to this work.

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Song, X., Hua, L., Xu, Y. et al. Involvement of RalB in the effect of geranylgeranyltransferase I on glioma cell migration and invasion. Clin Transl Oncol 17, 477–485 (2015). https://doi.org/10.1007/s12094-014-1263-x

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  • DOI: https://doi.org/10.1007/s12094-014-1263-x

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