Abstract
Nucleic acid sequencing is one of the most important tools of biological research with very broad application. Four generations of DNA sequencing technologies can be distinguished by their nature and the kind of output they provide. Sanger sequencing dominated for 30 years and was the workhorse of the Human Genome Project. In 2005 the first 2nd generation sequencer was presented with an output orders of magnitude higher than Sanger sequencing and dramatically reduced cost per base. Currently, we are at the dawn of third generation with nanopore systems that are being developed for DNA sequencing. Meanwhile, the field is broadening applications that complement first, second and third generation sequencing systems to get high-resolution genetic information and fourth generation sequencing is on the horizon. Second generation nucleic acid sequencers are systematically applied in many large-scale international projects such as the International Cancer Genome Consortium and the International Human Epigenome Project.
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References
Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA. 1977;74(12):5463–7.
Kircher M, Kelso J. High-throughput DNA sequencing—concepts and limitations. BioEssays. 2010;32(6):524–36.
International Human Genome Sequencing, C. Finishing the euchromatic sequence of the human genome. Nature. 2004;431(7011):931–45.
McGinn S, Gut IG. DNA sequencing—spanning the generations. N Biotechnol. 2013;30(4):366–72.
Maxam AM, Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci USA. 1977;74(2):560–4.
Metzker ML. Sequencing technologies—the next generation. Nat Rev Genet. 2010;11(1):31–46.
Margulies M, Egholm M, Altman WE, Attiya S, Bader JS, Bemben LA, et al. Genome sequencing in microfabricated high-density picolitre reactors. Nature. 2005;437(7057):376–80.
Bentley DR, Balasubramanian S, Swerdlow HP, Smith GP, Milton J, Brown CG, et al. Accurate whole human genome sequencing using reversible terminator chemistry. Nature. 2008;456(7218):53–9.
Valouev AJ, Ichikawa T, Tonthat J, Stuart S, Ranade S, Peckam H, et al. A high-resolution, nucleosome position map of C. elegans reveals a lack of universal sequence-dictated positioning. Genome Res. 2008;18(7):1051–63.
Rothberg JM, Hinz JM, Rearick TM, Schultz J, Mileski W, Davey M, et al. An integrated semiconductor device enabling non-optical genome sequencing. Nature. 2011;475(7356):348–52.
Schadt EE, Turner S, Kasarskis A. A window into third-generation sequencing. Hum Mol Genet. 2010;19(R2):R227–40.
Eid J, Fehr A, Gray J, Luong K, Lyle J, Otto G, et al. Real-time DNA sequencing from single polymerase molecules. Science. 2009;323(5910):133–8.
Clarke J, Wu HC, Jayasinghe L, Patel A, Reid S, Bayley H. Continuous base identification for single-molecule nanopore DNA sequencing. Nat Nanotechnol. 2009;4(4):265–70.
Larsson C, Grundberg I, Soderberg O, Nilsson M. In situ detection and genotyping of individual mRNA molecules. Nat Methods. 2010;7(5):395–7.
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The paper was written and funded through the European Community’s Seventh Framework Programme (FP7/2007-2013) under grant agreement no. [HEALTH-F4-2008-201418] entitled READNA.
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Gut, I.G. New sequencing technologies. Clin Transl Oncol 15, 879–881 (2013). https://doi.org/10.1007/s12094-013-1073-6
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DOI: https://doi.org/10.1007/s12094-013-1073-6