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Increased expression of CD24 is associated with tumor progression and prognosis in patients suffering osteosarcoma

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Abstract

Objective

As a small heavily glycosylated mucin-like glycosyl-phosphatidylinositol-anchored cell surface protein, CD24 plays an important role in carcinogenesis of various human malignancies. However, its involvement in osteosarcoma is still unclear. The aim of this study was to investigate the expression pattern and the clinical significance of CD24 in human osteosarcoma.

Methods

CD24 mRNA and protein expression levels were, respectively, detected by RT-PCR and Western blot assays using 30 pairs of osteosarcoma and noncancerous bone tissues. Then, immunohistochemistry was performed to analyze the association of CD24 expression in 166 osteosarcoma tissues with clinicopathological factors or survival of patients.

Results

CD24 expression at mRNA and protein levels were both significantly higher in osteosarcoma tissues than those in corresponding noncancerous bone tissues (both P < 0.001). In addition, CD24 protein was positively expressed in 129 of 166 (77.7 %) osteosarcoma specimens with a cytoplasmic and membraneous staining, and also increased in the osteosarcoma specimens with advanced clinical stage (P = 0.01) and positive distant metastasis (P = 0.005). The univariate and multivariate analyses showed that osteosarcoma patients with high CD24 expression had poorer overall and disease-free survival, and high CD24 expression was an independent prognostic factor for both overall and disease-free survival.

Conclusion

The aforementioned findings offer convincing evidence for the first time that the increased expression of CD24 is correlated with tumor aggressiveness and tumor metastasis of osteosarcoma, and this molecule is an independent prognostic marker for osteosarcoma patients.

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Conflict of interest

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Author information

Authors and Affiliations

  1. Orthopedics Department, Xuhui Central Hospital, No. 966, Middle Huaihai Road, Shanghai, 200031, China

    J. Tang & H. Cai

  2. Orthopedics Department, Zhujiang Hospital, The Southern Medical University, No. 253, Gongye Road, Haizhu District, Guangzhou, 510282, China

    L. Lin

  3. Orthopaedics Department, Shanghai East Hospital Tongji University, No. 150, Jimo Road, Shanghai, 200120, China

    P. Xie

  4. Shanghai Changhai Hospital Second Military Medical University, No. 168, Changhai Road, Shanghai, 200433, China

    W. Zhong

  5. Orthopaedics Department, Shanghai Sixth People’s Hospital, Shanghai Jiaotong University, No. 600, Yishan Road, Shanghai, 200233, China

    M. Tang

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  1. J. Tang
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  2. H. Cai
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  3. L. Lin
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  4. P. Xie
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  5. W. Zhong
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  6. M. Tang
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Corresponding author

Correspondence to M. Tang.

Additional information

J. Tang, H. Cai and L. Lin contributed equally to this article.

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Tang, J., Cai, H., Lin, L. et al. Increased expression of CD24 is associated with tumor progression and prognosis in patients suffering osteosarcoma. Clin Transl Oncol 15, 541–547 (2013). https://doi.org/10.1007/s12094-012-0961-5

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  • DOI: https://doi.org/10.1007/s12094-012-0961-5

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