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Decreased expression of chromodomain helicase DNA-binding protein 5 is an unfavorable prognostic marker in patients with primary gallbladder carcinoma

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Abstract

Aim

Chromodomain helicase DNA-binding protein 5 (CHD5) plays a role in normal neural development and in tumorigenesis of various human cancers. However, its role in primary gallbladder carcinoma (PGC) is still unclear. The aim of this study was to investigate CHD5 expression in PGC and its clinical significance.

Methods

CHD5 mRNA and protein expression in 120 PGC and 20 normal gallbladder specimens was determined by quantitative reverse transcription-polymerase chain reaction (QRT-PCR) and Western blotting analysis, respectively.

Results

The expression levels of CHD5 mRNA and protein in PGC tissues were both significantly lower than those in the normal epithelium of the gallbladder (mRNA: P = 0.006; protein: P = 0.01). CHD5 mRNA expression was closely correlated with its protein expression (r = 0.8; P < 0.001). Additionally, the low expression of CHD5 protein was significantly associated with high pathologic T stage (P = 0.01) and clinical stage (P = 0.008), and advanced histologic grade (P = 0.009). The expression levels of CHD5 protein in PGC tissues with positive nodal metastasis were also significantly lower than those without (P = 0.01). Survival analysis showed that low CHD5 expression was associated with shorter disease-free (P = 0.01) and overall survival (P = 0.008) compared to those with high CHD5 expression in PGC patients. Furthermore, multivariate analyses showed that the decreased expression of CHD5 was an independent prognostic marker for both unfavorable disease-free (P = 0.01) and overall survival (P = 0.006).

Conclusion

CHD5 may be involved in carcinogenesis of PGC and its down-regulation may be significantly correlated with unfavorable clinicopathologic features including poor overall and disease-free survival in patients.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (no.81172287).

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Authors and Affiliations

  1. Department of General Surgery, Tangdu Hospital, Fourth Military Medical University, 710038, Xi’an, People’s Republic of China

    Xilin Du, Tao Wu, Jianguo Lu, Li Zang, Nuan Song, Tao Yang, Huadong Zhao & Shengzhi Wang

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  1. Xilin Du
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  2. Tao Wu
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  3. Jianguo Lu
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  4. Li Zang
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  5. Nuan Song
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  6. Tao Yang
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  7. Huadong Zhao
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  8. Shengzhi Wang
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Corresponding authors

Correspondence to Xilin Du or Huadong Zhao.

Additional information

X. Du and T. Wu contributed equally to this work.

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Du, X., Wu, T., Lu, J. et al. Decreased expression of chromodomain helicase DNA-binding protein 5 is an unfavorable prognostic marker in patients with primary gallbladder carcinoma. Clin Transl Oncol 15, 198–204 (2013). https://doi.org/10.1007/s12094-012-0903-2

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  • DOI: https://doi.org/10.1007/s12094-012-0903-2

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