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APC gene deletions in gastric adenocarcinomas in a Chinese population: a correlation with tumour progression

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Abstract

Introduction

The adenomatous polyposis coli (APC) gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It has been shown to be involved in genetic instability and to be down-regulated in several human carcinomas. The chromosome locus of APC, 5q21-22, is frequently deleted in gastric cancers (GCs). The functional impact of such regions needs to be extensively investigated in large amount of clinical samples.

Patients and materials

Case-matched tissues of GC and adjacent normal epithelium (n=141) were included in this study. Quantitative PCR was carried out to examine the copy number as well as mRNA expression of APC gene in gastric malignancies.

Results

Our results showed that copy number deletions of APC were present in a relatively high percentage (25.9%, 34 out of 131) of gastric cancer samples. There was a correlation between APC deletion and tumor progression (p<0.01) as well as gene expression (p<0.05) in collected GC samples. On the other hand, mRNA levels of APC were also impaired in GC samples with unaltered copy numbers.

Conclusion

Sporadic GCs exhibit different mechanisms of APC regulation.

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Authors and Affiliations

  1. Biomedical Research Institute Shenzhen-PKU-HKUST Medical Center Guangdong Province, Shenzhen, People’s Republic of China

    Zhengyu Fang, Yi Xiong, Jiana Li, Li Liu, Chao Zhang & Jun Wan

  2. JNU-HKUST Joint Lab Ji-Nan University, Guangdong, People’s Republic of China

    Wei Zhang

  3. Section of Biochemistry and Cell Biology, Division of Life Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China

    Jun Wan

Authors
  1. Zhengyu Fang
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  2. Yi Xiong
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  3. Jiana Li
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  4. Li Liu
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  5. Wei Zhang
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  6. Chao Zhang
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  7. Jun Wan
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Corresponding author

Correspondence to Jun Wan.

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These authors contributed equally to this manuscript.

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Fang, Z., Xiong, Y., Li, J. et al. APC gene deletions in gastric adenocarcinomas in a Chinese population: a correlation with tumour progression. Clin Transl Oncol 14, 60–65 (2012). https://doi.org/10.1007/s12094-012-0762-x

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  • DOI: https://doi.org/10.1007/s12094-012-0762-x

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