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Vitrification: an effective new approach to oocyte banking and preserving fertility in cancer patients

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Abstract

Introduction

Oocyte cryopreservation is a useful tool for preserving the fertility of cancer patients at risk of losing ovarian function due to undergoing potentially sterilising therapies. Results obtained with different cryopreservation protocols have been disappointing, particularly those obtained with slow cooling procedures. The efficacy of vitrification as an application in clinical practice has recently been demonstrated. The aim of this study is to report results obtained with the Cryotop method of oocyte vitrification in a population of healthy women and to point out its potential usefulness for fertility preservation in oncological patients.

Materials and methods

The study population consisting of non-oncological patients included 47 oocyte donors and 57 recipients undergoing an oocyte donation cycle of assisted reproductive technology (ART). A total of 693 mature metaphase II oocytes were collected following ovarian stimulation using long protocol down-regulation plus gonadotropin administration. Vitrification was carried out by means of the Cryotop method. Oocytes were donated to a compatible recipient after endometrial preparation.

Results

Of the 693 oocytes, 666 (96.1%) survived. A total of 487 (73.1%) were fertilised successfully. One hundred and seventeen embryos were transferred to 57 recipients. Pregnancy rate per transfer and implantation rates were 63.2% and 38.5% respectively. Twenty-eight healthy babies were later born.

Conclusions

Oocyte cryo-banking by means of the Cryotop vitrification method represents a viable option for healthy women, producing excellent survival rates and a clinical outcome similar to that obtained with fresh oocytes. This approach could potentially be used in cancer patients who want to safeguard their fertility. Cancer patients could potentially benefit from this approach by storing their oocytes before the onset of the oncological therapy.

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References

  1. Akar M, Oktay K (2005) Restoration of ovarian endocrine function by ovarian transplantation. Trends Endocrinol Metab 16:374–380

    Article  PubMed  CAS  Google Scholar 

  2. Lee SJ, Schover LR, Partridge AH et al (2006) American Society of Clinical Oncology recommendations on fertility preservation in cancer patients. J Clin Oncol 24:2917–2931

    Article  PubMed  Google Scholar 

  3. Wallace WH, Anderson RA, Irvine DS (2005) Fertility preservation for young patients with cancer: who is at risk and what can be offered? Lancet Oncol 6:209–218

    Article  PubMed  Google Scholar 

  4. Apperley JF, Reddy N (1995) Mechanism and management of treatment-related gonadal failure in recipients of high dose chemoradiotherapy. Blood Rev 9:93–116

    Article  PubMed  CAS  Google Scholar 

  5. Yap JK, Davies M (2007) Fertility preservation in female cancer survivors. J Obstet Gynaecol 27:390–400

    Article  PubMed  CAS  Google Scholar 

  6. Sanchez M, Alama P, Gadea B et al (2007) Fresh human orthotopic ovarian cortex transplantation: long-term results. Hum Reprod 22:786–791

    Article  PubMed  CAS  Google Scholar 

  7. Oktay K, Buyuk E, Veeck L et al (2004) Embryo development after heterotopic transplantation of cryopreserved ovarian tissue. Lancet 363:837–840

    Article  PubMed  Google Scholar 

  8. Oktay K, Karlikaya G (2000) Ovarian function after transplantation of frozen, banked autologous ovarian tissue. N Engl J Med 342:1919

    Article  PubMed  CAS  Google Scholar 

  9. Mandelbaum J, Belaisch-Allart J, Junca AM et al (1998) Cryopreservation in human assisted reproduction is now routine for embryos but remains a research procedure for oocytes. Hum Reprod 13[Suppl 3]:161–174; discussion 175–177

    PubMed  Google Scholar 

  10. Wang JG, Douglas NC, Dicken C et al (2007) Cryopreservation of supernumerary high quality embryos predicts favorable outcomes for patients undergoing repeated cycles of in vitro fertilization. Fertil Steril 89:368–374

    Article  PubMed  Google Scholar 

  11. Freemann L, Trounson A, Kirby C (1986) Cryopreservation of human embryos: progress on the clinical use of the technique in human in vitro fertilization. J In Vitro Fert Embryo Transf 3:53–61

    Article  PubMed  CAS  Google Scholar 

  12. Oktay K, Buyuk E, Libertella N et al (2005) Fertility preservation in breast cancer patients: a prospective controlled comparison of ovarian stimulation with tamoxifen and letrozole for embryo cryopreservation. J Clin Oncol 23:4347–4353

    Article  PubMed  CAS  Google Scholar 

  13. Kuwayama M, Vajta G, Kato O, Leibo SP (2005) Highly efficient vitrification method for cryopreservation of human oocytes. Reprod Biomed Online 11:300–308

    PubMed  Google Scholar 

  14. Cobo A, Kuwayama M, Perez S et al (2007) Comparison of concomitant outcome achieved with fresh and cryopreserved donor oocytes vitrified by the Cryotop method. Fertil Steril, Epub ahead of print

  15. Soares SR, Troncoso C, Bosch E et al (2005) Age and uterine receptiveness: predicting the outcome of oocyte donation cycles. J Clin Endocrinol Metab 90:4399–4404

    Article  PubMed  CAS  Google Scholar 

  16. Remohi J, Gutierrez A, Cano F et al (1995) Long oestradiol replacement in an oocyte donation programme. Hum Reprod 10:1387–1391

    PubMed  CAS  Google Scholar 

  17. Woods EJ, Benson JD, Agca Y, Critser JK (2004) Fundamental cryobiology of reproductive cells and tissues. Cryobiology 48:146–156

    Article  PubMed  CAS  Google Scholar 

  18. Mazur P (2004) Principles of cryobiology. In: Fuller B, Lane M, Benson E (eds) Life in the frozen state. CRC Press, New York, pp 4–55

    Google Scholar 

  19. Oktay K, Cil AP, Bang H (2006) Efficiency of oocyte cryopreservation: a meta-analysis. Fertil Steril 86:70–80

    Article  PubMed  Google Scholar 

  20. Vajta G, Nagy ZP (2006) Are programmable freezers still needed in the embryo laboratory? Review on vitrification. Reprod Biomed Online 12:779–796

    PubMed  Google Scholar 

  21. Liebermann J, Dietl J, Vanderzwalmen P, Tucker MJ (2003) Recent developments in human oocyte, embryo and blastocyst vitrification: where are we now? Reprod Biomed Online 7:623–633

    PubMed  Google Scholar 

  22. Vajta G, Kuwayama M (2006) Improving cryopreservation systems. Theriogenology 65:236–244

    Article  PubMed  CAS  Google Scholar 

  23. Kuwayama M, Cobo A, Vajta G, (2007) Vitrification of oocytes: general considerations and the use of Cryotop method. In: Vitrification in assisted reproduction. A user’s manual and trouble shooting guide. Tucker M, Liebermann J eds. Informa health care 119:128

  24. Martino A, Songsasen N, Leibo SP (1996) Development into blastocysts of bovine oocytes cryopreserved by ultra-rapid cooling. Biol Reprod 54:1059–1069

    Article  PubMed  CAS  Google Scholar 

  25. Liebermann J, Nawroth F, Isachenko V et al (2002) Potential importance of vitrification in reproductive medicine. Biol Reprod 67:1671–1680

    Article  PubMed  CAS  Google Scholar 

  26. Lane M, Gardner DK (2001) Vitrification of mouse oocytes using a nylon loop. Mol Reprod Dev 58:342–347

    Article  PubMed  CAS  Google Scholar 

  27. Matsumoto H, Jiang JY, Tanaka T et al (2001) Vitrification of large quantities of immature bovine oocytes using nylon mesh. Cryobiology 42:139–144

    Article  PubMed  CAS  Google Scholar 

  28. Papis K, Shimizu M, Izaike Y (2000) Factors affecting the survivability of bovine oocytes vitrified in droplets. Theriogenology 54:651–658

    Article  PubMed  CAS  Google Scholar 

  29. Vajta G, Holm P, Kuwayama M et al (1998) Open Pulled Straw (OPS) vitrification: a new way to reduce cryoinjuries of bovine ova and embryos. Mol Reprod Dev 51:53–58

    Article  PubMed  CAS  Google Scholar 

  30. Kuwayama M (2007) Highly efficient vitrification for cryopreservation of human oocytes and embryos: the Cryotop method. Theriogenology 67:73–80

    Article  PubMed  CAS  Google Scholar 

  31. Katayama KP, Stehlik J, Kuwayama M et al (2003) High survival rate of vitrified human oocytes results in clinical pregnancy. Fertil Steril 80:223–224

    Article  PubMed  Google Scholar 

  32. Ruvalcaba L, Marínez R, Cuneo S et al (2005) Improving donor programs with an oocyte bank using vitrification. Fertil Steril 84[Suppl 1]:S70

    Article  Google Scholar 

  33. Lucena E, Bernal DP, Lucena C et al (2006) Successful ongoing pregnancies after vitrification of oocytes. Fertil Steril 85:108–111

    Article  PubMed  Google Scholar 

  34. Antinori M, Licata E, Dani G et al (2007) Cryotop vitrification of human oocytes results in high survival rate and healthy deliveries. Reprod Biomed Online 14:72–79

    Article  PubMed  Google Scholar 

  35. Bianchi V, Coticchio G, Distratis V et al (2007) Differential sucrose concentration during dehydration (0.2 mol/l) and rehydration (0.3 mol/l) increases the implantation rate of frozen human oocytes. Reprod Biomed Online 14:64–71

    Article  PubMed  CAS  Google Scholar 

  36. Levi Setti PE, Albani E, Novara PV et al (2006) Cryopreservation of supernumerary oocytes in IVF/ICSI cycles. Hum Reprod 21:370–375

    Article  PubMed  CAS  Google Scholar 

  37. Yoon TK, Kim TJ, Park SE et al (2003) Live births after vitrification of oocytes in a stimulated in vitro fertilization-embryo transfer program. Fertil Steril 79:1323–1326

    Article  PubMed  Google Scholar 

  38. Liebermann J, Tucker MJ, Sills ES (2003) Cryoloop vitrification in assisted reproduction: analysis of survival rates in > 1000 human oocytes after ultra-rapid cooling with polymer augmented cryoprotectants. Clin Exp Obstet Gynecol 30:125–129

    PubMed  CAS  Google Scholar 

  39. Selman H, Angelini A, Barnocchi N et al (2006) Ongoing pregnancies after vitrification of human oocytes using a combined solution of ethylene glycol and dimethyl sulfoxide. Fertil Steril 86:997–1000

    Article  PubMed  Google Scholar 

  40. Platet N, Cathiard AM, Gleizes M, Garcia M (2004) Estrogens and their receptors in breast cancer progression: a dual role in cancer proliferation and invasion. Crit Rev Oncol Hematol 51:55–67

    Article  PubMed  Google Scholar 

  41. García-Velasco JA, Moreno L, Pacheco A et al (2005) The aromatase inhibitor letrozole increases the concentration of intraovarian androgens and improves in vitro fertilization outcome in low responder patients: a pilot study. Fertil Steril 84:82–87

    Article  PubMed  CAS  Google Scholar 

  42. Oktay K, Hourvitz A, Sahin G et al (2006) Letrozole reduces estrogen and gonadotropin exposure in women with breast cancer undergoing ovarian stimulation before chemotherapy. J Clin Endocrinol Metab 91:3885–3890

    Article  PubMed  CAS  Google Scholar 

  43. Venn A, Watson L, Bruinsma F et al (1999) Risk of cancer after use of fertility drugs with in-vitro fertilisation. Lancet 354:1586–1590

    Article  PubMed  CAS  Google Scholar 

  44. Sonmezer M, Akar ME, Oktay K (2005) Strong family history in women who were diagnosed with breast cancer after in vitro fertilization. Fertil Steril 84[Suppl 1]:233–234

    Article  Google Scholar 

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Authors and Affiliations

  1. IVF Laboratory, IVI C/ Policía Local, 3, ES-46015, Valencia, Spain

    Ana Cobo, Javier Domingo, Sonia Pérez, Juana Crespo, José Remohí & Antonio Pellicer

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  1. Ana Cobo
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  2. Javier Domingo
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  3. Sonia Pérez
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  4. Juana Crespo
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  5. José Remohí
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  6. Antonio Pellicer
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Correspondence to Ana Cobo.

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Cobo, A., Domingo, J., Pérez, S. et al. Vitrification: an effective new approach to oocyte banking and preserving fertility in cancer patients. Clin Transl Oncol 10, 268–273 (2008). https://doi.org/10.1007/s12094-008-0196-7

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  • DOI: https://doi.org/10.1007/s12094-008-0196-7

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