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Characterization and Whole Genome Sequencing of Chromobacterium violaceum OUAT_2017: A Zoonotic Pathogen Found Fatal to a Wild Asiatic Elephant

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Abstract

This study reports a rare fatal case of Chromobacterium violeceum OUAT_2017 strain infection in an Asiatic elephant calf in India. Necropsy revealed pus-filled nodules in liver, spleen, and lungs. Nutrient broth cultures of nodule content showed sediment of violet pigment whereas smooth, non-diffusible, violet-pigmented, homogeneous colonies appeared on nutrient agar. The organism was found to be non-haemolytic and resistant to 8 of the 24 antibiotics tested in vitro. Partial 16S rRNA gene sequence measuring 1410 bp revealed 97% homology with C. violeceum. The bacterial genome composed of 64.87% of G + C content with total size of 4,681,202 bp. The genome annotation has 42 genes responsible for multidrug antibiotic resistance with the presence of Aminoglycoside-modifying enzymes (AAC (6’)) that targets streptomycin and spectinomycin. Our findings corroborated the lethal effect of C. violeceum in a new host (elephant) that enriched scientific information on epidemiological picture and whole genome sequencing as well.

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Acknowledgements

This study was supported by the officers, Department of Forests and Environment, Government of Odisha working in the concerned area.

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Authors and Affiliations

  1. Fisheries and Animal Resources Development Department, Government of Odisha, Bhubaneswar, Odisha, India

    Sourav R. Hota

  2. Centre for Wildlife Health, College of Veterinary Science and Animal Husbandry, Odisha University of Agriculture and Technology, Bhubaneswar, Odisha, 751003, India

    Soumesh K. Padhi, Avishek Pahari, Bikash K. Behera & Niranjana Sahoo

  3. Department of Epidemiology and Preventive Medicine, College of Veterinary Science and Animal Husbandry, Odisha University of Agriculture and Technology, Bhubaneswar, Odisha, 751003, India

    Biswaranjan Panda

  4. College of Veterinary Medicine, University of Minnesota, 1333 Gortner Ave, Saint Paul, Minnesota, 55108-1098, USA

    Sunil K. Mor, Vikash K. Singh & Sagar M. Goyal

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  1. Sourav R. Hota
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Correspondence to Niranjana Sahoo.

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Hota, S.R., Padhi, S.K., Pahari, A. et al. Characterization and Whole Genome Sequencing of Chromobacterium violaceum OUAT_2017: A Zoonotic Pathogen Found Fatal to a Wild Asiatic Elephant. Indian J Microbiol 62, 627–633 (2022). https://doi.org/10.1007/s12088-022-01047-4

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