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Influence of Metal Ions on the Immobilization of β-Glucosidase Through Protein-Inorganic Hybrids

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Abstract

Immobilization of enzymes through metal-based system is demonstrated as a promising approach to enhance its properties. In this study, the influence of metals ions, including copper, cobalt and zinc (Zn) on the immobilization of β-glucosidase (BGL) through the synthesis of protein-inorganic hybrid was evaluated at 4 °C. Among these metal ions-based hybrids, Zn showed the highest encapsulation yield and relative activity of 87.5 and 207%, respectively. Immobilized BGL exhibited higher pH and temperature stability compared to free form. Thermal stability of hybrid improved up to 26-fold at 60 °C. After 10 cycles of reuse, immobilized enzyme retained 93.8% of residual activity. These results suggested that metal ions played a significant role in the enzyme immobilization as a protein-inorganic hybrid. Overall, this strategy can be potentially applied to enhance the properties of enzymes though effective encapsulation for the broad biotechnological applications.

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Acknowledgements

This paper was supported by Konkuk University Researcher Fund in 2018. This research was supported by Basic Science Research Program (2019R1C1C1009766) and by the Intelligent Synthetic Biology Center of Global Frontier Project (2013M3A6A8073184) through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning. This research was supported by the KU Research Professor program of Konkuk University.

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Authors and Affiliations

  1. Division of Chemical Engineering, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul, 05029, Republic of Korea

    Sanjay K. S. Patel, Rahul K. Gupta, Virendra Kumar, Primata Mardina, Rowina Lestari, Vipin C. Kalia, Myung-Seok Choi & Jung-Kul Lee

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  1. Sanjay K. S. Patel
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  2. Rahul K. Gupta
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  3. Virendra Kumar
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Correspondence to Myung-Seok Choi or Jung-Kul Lee.

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Patel, S.K.S., Gupta, R.K., Kumar, V. et al. Influence of Metal Ions on the Immobilization of β-Glucosidase Through Protein-Inorganic Hybrids. Indian J Microbiol 59, 370–374 (2019). https://doi.org/10.1007/s12088-019-00796-z

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