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Annotation and De Novo Sequence Characterization of Extracellular β-Fructofuranosidase from Penicillium chrysogenum Strain HKF42

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Abstract

The genome of a fungal strain Penicillium chrysogenum strain HKF42, which can grow on 20% sucrose has been annotated for 7595 protein coding sequences. On mining of CAZymes, we could annotate a β-fructofuranosidase gene responsible for fructo-oligosaccharides (FOS) synthesis which is a known prebiotic. The enzyme activity was demonstrated and validated with the generation of FOS as kestose and nystose.

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Acknowledgements

Authors would like to acknowledge to CSIR-NEERI for providing essential resources for the research work [KRC No. CSIR-NEERI/KRC/2017/SEP/EBGD/2]. Vaibhav Gujar is thankful to University Grants Commission (UGC), New Delhi for providing Junior and Senior Research Fellowship for carrying out this research.

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Authors and Affiliations

  1. CSIR-National Environmental Engineering Research Institute (CSIR-NEERI), Nagpur, India

    Vaibhav V. Gujar, Priya Fuke, Anshuman A. Khardenavis & Hemant J. Purohit

  2. Academy of Scientific and Innovative Research (AcSIR), CSIR-NEERI, Nagpur, 440020, India

    Vaibhav V. Gujar & Anshuman A. Khardenavis

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  1. Vaibhav V. Gujar
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  2. Priya Fuke
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  3. Anshuman A. Khardenavis
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  4. Hemant J. Purohit
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Correspondence to Anshuman A. Khardenavis.

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Gujar, V.V., Fuke, P., Khardenavis, A.A. et al. Annotation and De Novo Sequence Characterization of Extracellular β-Fructofuranosidase from Penicillium chrysogenum Strain HKF42. Indian J Microbiol 58, 227–233 (2018). https://doi.org/10.1007/s12088-017-0704-y

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