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Knockout of pprM Decreases Resistance to Desiccation and Oxidation in Deinococcus radiodurans

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Abstract

Deinococcus radiodurans has attracted a great interest in the past decades due to its extraordinary resistance to ionizing radiation and highly efficient DNA repair system. Recent studies indicated that pprM is a putative pleiotropic gene in D. radiodurans and plays an important role in radioresistance and antioxidation, but its underlying mechanisms are poorly elucidated. In this study, pprM mutation was generated to investigate resistance to desiccation and oxidative stress. The result showed that the survival of pprM mutant under desiccation was markedly retarded compared to the wild strain from day 7–28. Furthermore, knockout of pprM increases the intercellular accumulation of ROS and the sensibility to H2O2 stress in the bacterial growth inhibition assay. The absorbance spectrum experiment for detecting the carotenoid showed that deinoxanthin, a carotenoid that peculiarly exists in Deinococcus, was reduced in the pprM mutant in the pprM mutant. Quantitative real time PCR showed decreased expression of three genes viz. CrtI (DR0861, 50%),CrtB (DR0862, 40%) and CrtO (DR0093, 50%), which are involved in deinoxanthin synthesis, and of Dps (DNA protection during starving) gene (DRB0092) relevant to ion combining and DNA protection in cells. Our results suggest that pprM may affect antioxidative ability of D. radiodurans by regulating the synthesis of deinoxanthin and the concentration of metal ions. This may provide new clues for the treatment of antioxidants.

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Acknowledgements

This work is supported by the Natural Science Foundation of China (Grant No. 81272993), and Program of Science and Technology Bureau of Hengyang city (Grant No. 2015KJ17). Graduate Student Research Innovation Project of University of South China (Grant No. 2016XCX39). The experiments were performed at the South University of China. We thank the efforts that all the colleges contributed to this work.

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  1. Yang Zeng and Yun Ma have contributed equally to this work.

Authors and Affiliations

  1. School of Public Health, University of South China, Hengyang, 421001, People’s Republic of China

    Yang Zeng, Fangzhu Xiao & Shuya He

  2. Department of Biochemistry and Biology, University of South China, Hengyang, Hunan, 421001, People’s Republic of China

    Yun Ma, Wuzhou Wang & Shuya He

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  1. Yang Zeng
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  2. Yun Ma
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Correspondence to Shuya He.

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Zeng, Y., Ma, Y., Xiao, F. et al. Knockout of pprM Decreases Resistance to Desiccation and Oxidation in Deinococcus radiodurans . Indian J Microbiol 57, 316–321 (2017). https://doi.org/10.1007/s12088-017-0653-5

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