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Comparison of DOT-ELISA and Standard-ELISA for Detection of the Vibrio cholerae Toxin in Culture Supernatants of Bacteria Isolated from Human and Environmental Samples

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Abstract

A comparison of DOT-ELISA and Standard-ELISA was made for detection of Vibrio cholerae toxin in culture supernatants of bacteria isolated from human and environmental samples. A total of 293 supernatants were tested in a double blind assay. A correlation of 100 % was obtained between both techniques. The cholera toxin was found in 20 Inaba and 3 Ogawa strains. Positive samples were from seafood (17 samples), potable water (1 sample) and sewage (5 samples). The DOT-ELISA was useful as the standard-ELISA to confirm the presence of cholera toxin in the environmental samples.

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Acknowledgments

We are grateful to D Solis-Hernández and RM Reyes-Díaz for technical attendance. The English was reviewed by Romel Hernández-Bello.

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Authors and Affiliations

  1. Coordinación de Investigaciones Inmunológicas, Instituto de Diagnóstico y Referencia Epidemiológicos, Secretaria de Salud, F. de P. Miranda 177, Lomas de Plateros, 01480, Ciudad de México, Mexico

    Antonio Meza-Lucas, Ricardo García-Rodea, Alejandro Escobar-Gutiérrez & Jorge-Luis de-la-Rosa-Arana

  2. Laboratorio de Producción de Sueros, Instituto de Diagnóstico y Referencia Epidemiológicos, Secretaria de Salud, Ciudad de México, Mexico

    María-Fernanda Pérez-Villagómez, José-Patricio Martínez-López, María-Guadalupe Martínez-Castelán & Altagracia Villanueva-Zamudio

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  1. Antonio Meza-Lucas
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  2. María-Fernanda Pérez-Villagómez
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  3. José-Patricio Martínez-López
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  6. Alejandro Escobar-Gutiérrez
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  7. Jorge-Luis de-la-Rosa-Arana
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  8. Altagracia Villanueva-Zamudio
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Correspondence to Jorge-Luis de-la-Rosa-Arana.

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Meza-Lucas, A., Pérez-Villagómez, MF., Martínez-López, JP. et al. Comparison of DOT-ELISA and Standard-ELISA for Detection of the Vibrio cholerae Toxin in Culture Supernatants of Bacteria Isolated from Human and Environmental Samples. Indian J Microbiol 56, 379–382 (2016). https://doi.org/10.1007/s12088-016-0596-2

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  • DOI: https://doi.org/10.1007/s12088-016-0596-2

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