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Identification and Characterization of Lactobacillus brevis P68 with Antifungal, Antioxidant and Probiotic Functional Properties

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Abstract

In the present study, Lactobacillus pentosus, L. plantarum, L. fermentum, L. brevis, L. paraplantarum, L. buchneri, and L. acidipiscis, which are strains capable of producing antifungal metabolites against food-spoilage fungi, were isolated and identified based on 16S rRNA gene sequencing from different traditional pickles. L. brevis P68 exhibited significant antifungal activity, and it’s in vitro antioxidant and probiotic properties were investigated. The antifungal compound was characterized based on 13C nuclear magnetic resonance (NMR), 1H NMR, infrared, and mass spectral data. The minimum inhibitory concentration (MIC) of the compounds was assessed using the broth micro-dilution technique. The MIC of the compounds against Penicillium chrysogenum and P. roqueforti was 2.5 mg mL−1 and that against Gibberella moniliformis and Aspergillus clavatus was 5.0 mg mL−1. In addition, the H2O2 (1.0 mM) hydroxyl radical, and DPPH scavenging activity inhibition rates were 32.76 and 48.63 %, respectively, and the activities toward the glutathione peroxidase and superoxide dismutase enzymes were high. This strain tolerated low pH and bile salt, exhibited bile salt hydrolase and extracellular enzyme activities, and was sensitive to common antibiotics with high hydrophobicity. This study revealed that the antifungal, antioxidant, and probiotic properties of L. brevis P68 confirmed its application to the food industry.

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Acknowledgments

This study was supported by the Deanship of Scientific Research, College of Science Research Centre, King Saud University, P. O. Box 2455, Riyadh 11451, Saudi Arabia.

Conflict of interest

The authors declare that there are no conflicts of interest associated with this work.

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Authors and Affiliations

  1. Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, P. O. Box 2455, Riyadh, 11451, Saudi Arabia

    Mariadhas Valan Arasu, Naif Abdullah Al-Dhabi, Veeramuthu Duraipandiyan & Ponmurugan Karuppiah

  2. Department of Zoology, Thiru Vika Government Arts College, Thiruvarur, 610 003, Tamil Nadu, India

    Thankappan Sarasam Rejiniemon & V. A. J. Huxley

  3. Department of Oriental Medicine Materials, Dongsin University, Naju, Republic of Korea

    Kyung Dong Lee

  4. The United Graduate School of Agricultural Sciences, Tottori University, Tottori-Shi, Japan

    Da Hye Kim

  5. Grassland and forage division, National Institute of Animal Science, RDA, Seonghwan-Eup, Cheonan-Si, Chungnam, 330-801, Republic of Korea

    Ki Choon Choi

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  1. Mariadhas Valan Arasu
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  2. Naif Abdullah Al-Dhabi
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  3. Thankappan Sarasam Rejiniemon
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  4. Kyung Dong Lee
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  5. V. A. J. Huxley
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  6. Da Hye Kim
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  7. Veeramuthu Duraipandiyan
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  8. Ponmurugan Karuppiah
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  9. Ki Choon Choi
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Correspondence to Ki Choon Choi.

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Arasu, M.V., Al-Dhabi, N.A., Rejiniemon, T.S. et al. Identification and Characterization of Lactobacillus brevis P68 with Antifungal, Antioxidant and Probiotic Functional Properties. Indian J Microbiol 55, 19–28 (2015). https://doi.org/10.1007/s12088-014-0495-3

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  • DOI: https://doi.org/10.1007/s12088-014-0495-3

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