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Characterization and Fungal Inhibition Activity of Siderophore from Wheat Rhizosphere Associated Acinetobacter calcoaceticus Strain HIRFA32

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Abstract

Acinetobacter calcoaceticus HIRFA32 from wheat rhizosphere produced catecholate type of siderophore with optimum siderophore (ca. 92 % siderophore units) in succinic acid medium without FeSO4 at 28 °C and 24 h of incubation. HPLC purified siderophore appeared as pale yellow crystals with molecular weight [M+1] m/z 347.18 estimated by LCMS. The structure elucidated by 1H NMR, 13C NMR, HMQC, HMBC, NOESY and decoupling studies, revealed that siderophore composed of 2,3-dihydroxybenzoic acid with hydroxyhistamine and threonine as amino acid subunits. In vitro study demonstrated siderophore mediated mycelium growth inhibition (ca. 46.87 ± 0.5 %) of Fusarium oxysporum. This study accounts to first report on biosynthesis of acinetobactin-like siderophore by the rhizospheric strain of A. calcoaceticus and its significance in inhibition of F. oxysporum.

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Acknowledgments

We thank Department of Biotechnology (DBT), Govt. of India, New Delhi (Project Sanction No. BT/PR6454/AGR/05/302/2005) for the financial support.

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Authors and Affiliations

  1. Institute of Bioinformatics and Biotechnology, University of Pune, Pune, 411007, India

    D. V. Maindad, H. Chaudhari & D. P. Sachdev

  2. Department of Chemistry, University of Pune, Pune, 411007, India

    V. M. Kasture & D. D. Dhavale

  3. Department of Microbiology, University of Pune, Pune, 411007, India

    B. A. Chopade

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  1. D. V. Maindad
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Correspondence to D. P. Sachdev.

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Maindad, D.V., Kasture, V.M., Chaudhari, H. et al. Characterization and Fungal Inhibition Activity of Siderophore from Wheat Rhizosphere Associated Acinetobacter calcoaceticus Strain HIRFA32. Indian J Microbiol 54, 315–322 (2014). https://doi.org/10.1007/s12088-014-0446-z

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