Abstract
Porcine reproductive and respiratory syndrome (PRRS) is considered one of the most important infectious diseases to affect the swine industry and characterized by reproductive failure in late term gestation in sows and respiratory disease in pigs of all ages. The GP5a gene, encoding RNA-dependent RNA polymerase, is generally regarded as fairly conserved when compared to other viral proteins. It plays an important role in the process of duplication and transcription carried out by Porcine reproductive and respiratory syndrome virus (PRRSV). We firstly expressed and purified the GP5a protein of PRRSV. This provides a good method for the purification of expressed proteins and the preparation of the corresponding antibodies.
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Acknowledgments
This work was supported by the National Natural Sciences Foundation of China (Nos. 31272564, 31201876), the Joint Fund of NSFC-Guangdong of China (U0931003), and the Modern Agricultural Industry Technology System (CARS-36).
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Chunya Wei and Zhen Huang contributed equally to this work.
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Wei, C., Huang, Z., Sun, L. et al. Expression and Antibody Preparation of GP5a Gene of Porcine Reproductive and Respiratory Syndrome Virus. Indian J Microbiol 53, 370–375 (2013). https://doi.org/10.1007/s12088-013-0368-1
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DOI: https://doi.org/10.1007/s12088-013-0368-1