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In Vitro Antibacterial Activity of Cysteine Protease Inhibitor from Kiwifruit (Actinidia deliciosa)

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Abstract

The need for replacing traditional pesticides with alternative agents for the management of agricultural pathogens is rising worldwide. In this study, a cysteine proteinase inhibitor (CPI), 11 kDa in size, was purified from green kiwifruit to homogeneity. We examined the growth inhibition of three plant pathogenic Gram-negative bacterial strains by kiwi CPI and attempted to elucidate the potential mechanism of the growth inhibition. CPI influenced the growth of phytopathogenic bacteria Agrobacterium tumefaciens (76.2 % growth inhibition using 15 μM CPI), Burkholderia cepacia (75.6 % growth inhibition) and, to a lesser extent, Erwinia carotovora (44.4 % growth inhibition) by inhibiting proteinases that are excreted by these bacteria. Identification and characterization of natural plant defense molecules is the first step toward creation of improved methods for pest control based on naturally occurring molecules.

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Acknowledgments

This work is supported by the Ministry of Science and Technological Development, Republic of Serbia, Grant No. 172049.

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Chemistry, University of Belgrade, Belgrade, Serbia

    Milica Popovic, Milica Grozdanovic & Marija Gavrovic-Jankulovic

  2. Institute of Virology, Vaccines and Sera Torlak, Belgrade, Serbia

    Ivana Aleksic

  3. Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Belgrade, Serbia

    Uros Andjelkovic

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  1. Milica Popovic
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  2. Uros Andjelkovic
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  3. Milica Grozdanovic
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  4. Ivana Aleksic
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  5. Marija Gavrovic-Jankulovic
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Correspondence to Milica Popovic.

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Popovic, M., Andjelkovic, U., Grozdanovic, M. et al. In Vitro Antibacterial Activity of Cysteine Protease Inhibitor from Kiwifruit (Actinidia deliciosa). Indian J Microbiol 53, 100–105 (2013). https://doi.org/10.1007/s12088-012-0319-2

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  • DOI: https://doi.org/10.1007/s12088-012-0319-2

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