Skip to main content
SpringerLink
Log in

Continuous Production of Dextran from Immobilized Cells of Leuconostoc mesenteroides KIBGE HA1 Using Acrylamide as a Support

  • Original Article
  • Published:
Indian Journal of Microbiology Aims and scope Submit manuscript

Abstract

The cells of L. mesenteroides KIBGE HA1 were immobilized for the production of dextran on acrylamide gel and gel concentration was optimized for maximum entrapment. Sucrose at substrate concentration of 10% produced high yield of dextran at 25°C with a percent conversion of 5.82 while at 35°C it was 3.5. However, increasing levels of sucrose diminished dextran yields. The free cells stopped producing dextran after 144 h, while immobilized cells continued to produce dextran even after 480 h. Molecular mass distribution of dextran from free cells indicate that it is identical to that of blue dextran while the molecular mass of dextran from immobilized cells is lower than that of free cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Farwa S, Qader SAU, Afsheen A, Nuzhat A (2008) Production & characterization of a unique dextran from an indigenous Leuconostoc mesenteroides CMG713. Int J Biol Sci 4(6):379–386

    Google Scholar 

  2. Jeanes A (1966) Dextran. In: Mark HF (ed) Encyclopedia of polymer science and technology, vol 4. Wiley, New York, pp 805–824

    Google Scholar 

  3. Monsan P, Bozonnet S, Albenne C, Joucla G, Willemot RM, Remaud-Simeon M (2001) Homopolysaccharides from lactic acid bacteria. Int Dairy J 11:675–685

    Article  CAS  Google Scholar 

  4. Sidebotham RL (1974) Dextrans. Adv Carbohydr Chem Biochem 30:371–444

    Article  PubMed  CAS  Google Scholar 

  5. Jeanes A, Haynes WC, Wilham CA, Rankin JC, Melvin EH, Austin MJ, Cluskey JE, Fisher BE, Tsuchiya HM, Rist CE (1954) Characterization and classification of dextrans from ninety-six strains of bacteria. J Am Chem Soc 76:5041–5052

    Article  CAS  Google Scholar 

  6. Reiter B, Oram JD (1982) Nutritional studies on cheese starter. 1. Vitamin and amino acid requirements of single strain starters. J Dairy Res 29:63–68

    Google Scholar 

  7. Dols M, Remaud-Simeon M, Monsan PF (1997) Dextransucrase production by Leuconostoc mesenteroides NRRL B-1299 comparison with L. mesenteroides NRRL B512F. Enzym Microb Technol 20:523–530

    Article  CAS  Google Scholar 

  8. Dols M, Willemot RM, Monsan PF, Remaud-Simeon M (2001) Factor affecting α-1,2 glucooligosaccharide synthesis by Leuconostoc mesenteroides NRRL B1299 dextransucrase. Biotechnol Bioeng 74:498–504

    Article  Google Scholar 

  9. Alcalde M, Plou FJ, Gomez de Segura A, Remaud-Simeon M, Willemot RM, Monsan P, Ballesteros A (1999) Immobilization of native and dextran free dextransucrase from Leuconostoc mesenteroides NRRL B512F for the synthesis of glucooligosaccharides. Biotechnol Tech 13:749–755

    Article  CAS  Google Scholar 

  10. Qader SAU, Afsheen A, Noman S, Saeeda B, Abid A (2007) Characterization of dextransucrase immobilized on calcium alginate beads from Leuconostoc mesenteroides PCSIR-4. Ital J Biochem 56(2):158–162

    CAS  Google Scholar 

  11. Ebert KH, Schenk G (1968) Mechanism of Biopolymer growth: the formation of dextran and levan. Adv Enzymol 30:179–221

    PubMed  CAS  Google Scholar 

  12. Robyt JF, Corrigan AJ (1977) The mechanism of dextransucrase action: activation of dextransucrase from Streptococcus mutans OMZ 176 by dextran and modified dextran and the nonexistence of the primer requirement for the synthesis of dextran. Arch Biochem Biophys 183:726–731

    Article  PubMed  CAS  Google Scholar 

  13. Robyt JF, Taniguchi H (1976) The mechanism of dextransucrase action. Biosynthesis of branch linkages by acceptor reactions with dextran. Arch Biochem Biophys 174(1):129–135

    Article  PubMed  CAS  Google Scholar 

  14. Qader SAU, Iqbal L, Rizvi HA, Zuberi R (2001) Production of dextran from sucrose by newly isolated strain of Leuconostoc mesenteroides (PCSIR-3) with reference to L. mesenteroides NRRL B-512F. Biotechnol Appl Biochem 34:93–97

    Article  Google Scholar 

  15. Martinez-Espindola JP, Lopez-Munguia CA (1985) The kinetics of dextransucrase and dextran synthesis in batch reactors. Biotechnol Lett 7:483–486

    Article  CAS  Google Scholar 

  16. Zedan HH, EL-Tayeb OM, Hshen AA (1983) A qualitative study of the production of dextran from sucrose by freshly isolated strains of Leuconostoc mesenteroides. Egypt J Microbiol Special Issue 47–65

  17. Lopez A, Monsan P (1980) Dextran synthesis by immobilized dextransucrase. Biochimie 62:323–329

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

We are also thankful to Miss Samina Iqbal and M. Hussain for isolation and identification of Leuconostoc mesenteroides KIBGE HA1.

Author information

Authors and Affiliations

  1. The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi, 75270, Pakistan

    Shah Ali Ul Qader, Afsheen Aman & Abid Azhar

Authors
  1. Shah Ali Ul Qader
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Afsheen Aman
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Abid Azhar
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Shah Ali Ul Qader.

Additional information

Gene Bank: Accession number FJ467346.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Qader, S.A.U., Aman, A. & Azhar, A. Continuous Production of Dextran from Immobilized Cells of Leuconostoc mesenteroides KIBGE HA1 Using Acrylamide as a Support. Indian J Microbiol 51, 279–282 (2011). https://doi.org/10.1007/s12088-011-0130-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12088-011-0130-5

Keywords

Search

Navigation