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Distribution of Putative Virulence Genes and Antimicrobial Drug Resistance in Vibrio harveyi

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Abstract

The marine-estuarine bacterium Vibrio harveyi is an important pathogen of invertebrates, most significantly, the larvae of commercially important shrimp Penaeus monodon. In this study, we analyzed V. harveyi isolated from shrimp hatchery environments for understanding the distribution of putative virulence genes and antimicrobial drug resistance. The putative genes targeted for PCR detection included four reversible toxin (Rtx)/hemolysin genes, a gene encoding homologue of Vibrio cholerae zonula occludens toxin (Zot) and a hemolysin-coregulated protein gene (hcp) by polymerase chain reaction (PCR). Of the four putative reversible toxin genes, vhh-1 was detected in 31% of the isolates, vhh-2 in 46%, vhh-3 in 23% and vhh-4 was detected in 27% of the isolates. A zot-like sequence of bacteriophage f237 was present in 15%, while hcp sequence was detected in 48% of the isolates. The antimicrobial susceptibility test revealed resistance to several groups of antibiotics including β-lactams, cephalosporins, macrolides, quinolones, nitrofurantoin and tetracycline.

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Acknowledgments

The authors are grateful to the Director, NIO, Goa and the Scientist- in- charge, NIO (RC), Cochin for their support and advice. Financial support from suprainstitutional project SIP 1302 is gratefully acknowledged. The authors thank Dr. Sanath Kumar, ENMU, USA for valuable suggestions and critical reading of the manuscript. Assistance by Mr. Kiran Krishna, Junior technical assistant, NIO (RC), Cochin is acknowledged. This is NIO contribution number 4559.

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Authors and Affiliations

  1. Molecular Biology Laboratory, National Institute of Oceanography, Regional Centre (CSIR), Dr. Salim Ali Road, P.O. Box 1913, Kochi, 682 018, India

    Ammini Parvathi, Dafini Mendez & Ciana Anto

  2. Department of Biotechnology, St. Joseph’s College, Irinjalakuda, India

    Dafini Mendez

  3. Department of Microbiology, Sree Sankara College, Kalady, India

    Ciana Anto

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  1. Ammini Parvathi
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  2. Dafini Mendez
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  3. Ciana Anto
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Correspondence to Ammini Parvathi.

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Parvathi, A., Mendez, D. & Anto, C. Distribution of Putative Virulence Genes and Antimicrobial Drug Resistance in Vibrio harveyi . Indian J Microbiol 51, 332–337 (2011). https://doi.org/10.1007/s12088-011-0128-z

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  • DOI: https://doi.org/10.1007/s12088-011-0128-z

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