Abstract
Regulated necrosis (necroptosis) plays a pivotal role in the extent of cardiomyocyte loss and the development of post-ischemic adverse remodelling and cardiac dysfunction following myocardial I/R injury. Although HIIT has been reported to give rise to cardioprotection against MI, but the detailed knowledge of its molecular targets for treatment of MI is still not available. The LAD of Male Wistar rats was occluded to induce MI for 30 min and reperfusion for eight weeks. We investigated the effect of long-term HIIT for eight weeks on lipid peroxidation, SOD activity and GSH content using ELISA assay. Cardiac function, fibrosis, and infarct size were assessed by echocardiography, Masson’s trichrome and Evans Blue/TTC dual staining respectively. The expressions of gene markers of myocardial hypertrophy, fibrosis and key mediators of necroptosis were measured using RT-PCR and western blotting assay respectively. The results indicated that HIIT reduced lipid peroxidation, infarct size and improved endogenous antioxidant system and heart function. Significant decreases in mRNA levels of procollagen α1(I), α1(III), and fibronectin1were observed following HIIT. Moreover, that HIIT significantly decreased the expression of key mediators of necroptosis induced by MI (P < 0.05). There were no significant differences in β-MHC mRNA level in different groups. The findings of study suggest that HIIT might exert cardioprotective effects against post-ischemic adverse remodeling through targeting necroptosis process. Likewise, cardioprotective effects of HIIT in coping with myocardial I/R injury may be associated with RIP1-RIP3-MLKL axis. These findings establish a critical foundation for higher efficiency of exercise-based cardiac rehabilitation post–MI and future research.
Similar content being viewed by others
Abbreviations
- MI:
-
Myocardial infarction
- I/R:
-
Ischemia/reperfusion
- ROS:
-
Reactive oxygen species
- RIP1:
-
Receptor-interacting protein kinase1
- NF-κB:
-
Nuclear factor-κB
- MLKL:
-
The mixed lineage kinase domain-like protein
- TRPM7:
-
Transient receptor potential melastatin related 7
- HIIT:
-
High-intensity interval training
- MICT:
-
Moderate intensity continuous training
- CExT:
-
Customary continuous exercise training
- LVIDd:
-
Left ventricular diameter in diastole
- LVIDs:
-
Left ventricular diameter in systole
- FS:
-
Fractional shortening
- EF:
-
Ejection fraction
- TBARS:
-
The thiobarbituric acid reactive substance
- TTC:
-
2,3,5- triphenyl-2H-tetrazolium chloride
- MDA:
-
Malondialdehyde
- LAD:
-
Left anterior descending coronary artery
- KCl:
-
Potassium chloride
- NBT:
-
Nitroblue tetrazolium
- GSH:
-
Reduced glutathione
- UCPs:
-
Uncoupling proteins
- eNOS:
-
Endothelial nitric oxide synthase
- CaMKII:
-
Ca2 + _calmodulin–dependent protein kinase
- LDH:
-
Lactate dehydrogenase
- CK:
-
Creatine kinase
- CMT:
-
Continuous Moderately Training
- SOD:
-
Superoxide dismutase
- GAPDH:
-
Glyceraldehyde-3-phosphate Dehydrogenase
- β-MHC:
-
Beta-myosin heavy chain
References
Ajami M, Davoodi SH, Habibey R, Namazi N, Soleimani M, Pazoki-Toroudi H (2013) Effect of DHA+ EPA on oxidative stress and apoptosis induced by ischemia-reperfusion in rat kidneys. Fundam Clin Pharmacol 27:593–602. https://doi.org/10.1111/j.1472-8206.2012.01066.x
Ajami M, Pazoki-Toroudi H, Amani H, Nabavi SF, Braidy N, Vacca RA, Atanasov AG, Mocan A, Nabavi SM (2016) Therapeutic role of sirtuins in neurodegenerative disease and their modulation by polyphenols. Neurosci Biobehav Rev 73:39–47. https://doi.org/10.1016/j.neubiorev.2016.11.022
Amani H, Ajami M, Maleki SN, Pazoki-Toroudi H, Daglia M, Sokeng AJT, Di Lorenzo A, Nabavi SF, Devi KP, Nabavi SM (2017a) Targeting signal transducers and activators of transcription (STAT) in human cancer by dietary polyphenolic antioxidants. Biochimie 142:63–79. https://doi.org/10.1016/j.biochi.2017.08.007
Amani H, Habibey R, Hajmiresmail SJ, Latifi S, Pazoki-Toroudi H, Akhavan O (2017b) Antioxidant nanomaterials in advanced diagnoses and treatments of ischemia reperfusion injuries. J Mater Chem B 5:9452–9476. https://doi.org/10.1039/C7TB01689A
Batacan RB, Duncan MJ, Dalbo VJ, Tucker PS, Fenning AS (2016) Effects of high-intensity interval training on cardiometabolic health: a systematic review and meta-analysis of intervention studies. In: Br J Sports Med: bjsports-2015-095841, vol 51, pp 494–503. https://doi.org/10.1136/bjsports-2015-095841
Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44:276–287. https://doi.org/10.1016/0003-2697(71)90370-8
Boyne AF, Ellman GL (1972) A methodology for analysis of tissue sulfhydryl components. Anal Biochem 46:639–653. https://doi.org/10.1016/0003-2697(72)90335-1
Brown MB, Neves E, Long G, Graber J, Gladish B, Wiseman A, Owens M, Fisher AJ, Presson RG, Petrache I (2016) High-intensity interval training, but not continuous training, reverses right ventricular hypertrophy and dysfunction in a rat model of pulmonary hypertension. Am J Phys Regul Integr Comp Phys 312:R197–R210. https://doi.org/10.1152/ajpregu.00358.2016
Cai Z, Jitkaew S, Zhao J, Chiang H-C, Choksi S, Liu J, Ward Y, Wu L-g, Liu Z-G (2014) Plasma membrane translocation of trimerized MLKL protein is required for TNF-induced necroptosis. Nat Cell Biol 16:55–65. https://doi.org/10.1038/ncb2883
Christofferson DE, Yuan J (2010) Necroptosis as an alternative form of programmed cell death. Curr Opin Cell Biol 22:263–268. https://doi.org/10.1016/j.ceb.2009.12.003
Chtourou Y, Slima AB, Makni M, Gdoura R, Fetoui H (2015) Naringenin protects cardiac hypercholesterolemia-induced oxidative stress and subsequent necroptosis in rats. Pharmacol Rep 67:1090–1097. https://doi.org/10.1016/j.pharep.2015.04.002
de Oliveira Sá G, dos Santos Neves V, de Oliveira Fraga SR, Souza-Mello V, Barbosa-da-Silva S (2017) High-intensity interval training has beneficial effects on cardiac remodeling through local renin-angiotensin system modulation in mice fed high-fat or high-fructose diets. Life Sci 189:8–17. https://doi.org/10.1016/j.lfs.2017.09.012
Declercq W, Vanden Berghe T, Vandenabeele P (2009) RIP kinases at the crossroads of cell death and survival. Cell 138:229–232. https://doi.org/10.1016/j.cell.2009.07.006
Ding R, Xia K, Hu D (2016) Study on the effect of different exercise intensity on cardiac function of rats with acute myocardial infarction and differential expression of circulating MiRNAs. In: Am Heart Assoc. Circulation 134(Suppl 1):A20726
Dondelinger Y, Hulpiau P, Saeys Y, Bertrand MJM, Vandenabeele P (2016) An evolutionary perspective on the necroptotic pathway. Trends Cell Biol 26:721–732. https://doi.org/10.1016/j.tcb.2016.06.004
Dorn GW (2008) Apoptotic and non-apoptotic programmed cardiomyocyte death in ventricular remodelling. Cardiovasc Res 81:465–473. https://doi.org/10.1093/cvr/cvn243
Esterbauer H, Cheeseman KH (1990) '[42] determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal. Methods Enzymol 186:407–421. https://doi.org/10.1016/0076-6879(90)86134-H
Fallahi AA, Shekarfroush S, Rahimi M, Jalali A, Khoshbaten A (2016) Alteration in cardiac uncoupling proteins and eNOS gene expression following high-intensity interval training in favor of increasing mechanical efficiency. Iran J Basic Med Sci 19(3):258–264
Garza MA, Wason EA, Zhang JQ (2015) Cardiac remodeling and physical training post myocardial infarction. World J Cardiol 7:52. PMID: 25717353–64
Ghadernezhad N, Khalaj L, Pazoki-Toroudi H, Mirmasoumi M, Ashabi G (2016) Metformin pretreatment enhanced learning and memory in cerebral forebrain ischaemia: the role of the AMPK/BDNF/P70SK signalling pathway. Pharm Biol 54:2211–2219. https://doi.org/10.3109/13880209.2016.1150306
Giallauria F, Acampa W, Ricci F, Vitelli A, Torella G, Lucci R, Del Prete G, Zampella E, Assante R, Rengo G (2013) Exercise training early after acute myocardial infarction reduces stress-induced hypoperfusion and improves left ventricular function. Eur J Nucl Med Mol Imaging 40:315–324. https://doi.org/10.1007/s00259-012-2302-x
Guiraud T, Nigam A, Gremeaux V, Meyer P, Juneau M, Bosquet L (2012) High-intensity interval training in cardiac rehabilitation. Sports Med 42:587–605. https://doi.org/10.2165/11631910-000000000-00000
Hallsworth K, Thoma C, Hollingsworth KG, Cassidy S, Anstee QM, Day CP, Trenell MI (2015) Modified high-intensity interval training reduces liver fat and improves cardiac function in non-alcoholic fatty liver disease: a randomized controlled trial. Clin Sci 129:1097–1105. https://doi.org/10.1042/CS20150308
Han CH, Guan ZB, Zhang PX, Fang HL, Li L, Zhang HM, Zhou FJ, Mao YF, Liu WW (2018) Oxidative stress induced necroptosis activation is involved in the pathogenesis of hyperoxic acute lung injury. Biochem Biophys Res Commun 495:2178–2183. https://doi.org/10.1016/j.bbrc.2017.12.100
Hochhauser E, Cheporko Y, Yasovich N, Pinchas L, Offen D, Barhum Y, Pannet H, Tobar A, Vidne BA, Birk E (2007) Bax deficiency reduces infarct size and improves long-term function after myocardial infarction. Cell Biochem Biophys 47:11–19. https://doi.org/10.1385/CBB:47:1:11
Jazayeri MH, Amani H, Pourfatollah AA, Avan A, Ferns GA, Pazoki-Toroudi H (2016a) Enhanced detection sensitivity of prostate-specific antigen via PSA-conjugated gold nanoparticles based on localized surface plasmon resonance: GNP-coated anti-PSA/LSPR as a novel approach for the identification of prostate anomalies. Cancer Gene Ther 23(10):365–369. https://doi.org/10.1038/cgt.2016.42
Jazayeri MH, Amani H, Pourfatollah AA, Pazoki-Toroudi H, Sedighimoghaddam B (2016b) Various methods of gold nanoparticles (GNPs) conjugation to antibodies. Sensing and bio-sensing research 9:17–22. https://doi.org/10.1016/j.sbsr.2016.04.002
Kaczmarek A, Vandenabeele P, Krysko DV (2013) Necroptosis: the release of damage-associated molecular patterns and its physiological relevance. Immunity 38:209–223. https://doi.org/10.1016/j.immuni.2013.02.003
Koshinuma S, Miyamae M, Kaneda K, Kotani J, Figueredo VM (2014) Combination of necroptosis and apoptosis inhibition enhances cardioprotection against myocardial ischemia–reperfusion injury. J Anesth 28:235–241. https://doi.org/10.1007/s00540-013-1716-3
Kraljevic J, Marinovic J, Pravdic D, Zubin P, Dujic Z, Wisloff U, Ljubkovic M (2013) Aerobic interval training attenuates remodelling and mitochondrial dysfunction in the post-infarction failing rat heart. Cardiovasc Res 99:55–64. https://doi.org/10.1093/cvr/cvt080
Levine GN, Bates ER, Bittl JA, Brindis RG, Fihn SD, Fleisher LA, Granger CB, Lange RA, Mack MJ, Mauri L (2016) 2016 ACC/AHA guideline focused update on duration of dual antiplatelet therapy in patients with coronary artery disease: a report of the American College of Cardiology/American Heart Association task force on clinical practice guidelines. J Thorac Cardiovasc Surg 152:1243–1275. https://doi.org/10.1016/j.jacc.2016.03.513
Li L, Chen Y, Doan J, Murray J, Molkentin JD, Liu Q (2014) A TAK1 signaling pathway critically regulates myocardial survival and remodeling. Circulation 130(24):2162–2172. https://doi.org/10.1161/CIRCULATIONAHA.114.011195
Li C, Xue H, Yang Z, Shi Z, Zhang B, Yu L, Ma H (2017) GW28-e0894 impaired autophagosome clearance triggers myocardial necroptosis in ischemia/reperfusion injury. J Am Coll Cardiol 70:C33. https://doi.org/10.1016/j.jacc.2017.07.114
Linkermann A, Bräsen JH, Himmerkus N, Liu S, Huber TB, Kunzendorf U, Krautwald S (2012) Rip1 (receptor-interacting protein kinase 1) mediates necroptosis and contributes to renal ischemia/reperfusion injury. Kidney Int 81:751–761. https://doi.org/10.1038/ki.2011.450
Luedde M, Lutz M, Carter N, Sosna J, Jacoby C, Vucur M, Gautheron J, Roderburg C, Borg N, Reisinger F (2014) RIP3, a kinase promoting necroptotic cell death, mediates adverse remodelling after myocardial infarction. Cardiovasc Res 103:206–216. https://doi.org/10.1093/cvr/cvu146
Mahmoudi M, Yu M, Serpooshan V, Wu JC, Langer R, Lee RT, Karp JM, Farokhzad OC (2017) Multiscale technologies for treatment of ischemic cardiomyopathy. Nat Nanotechnol 12:845. https://doi.org/10.1038/nnano.2017.167
Mair J, Wagner I, Jakob G, Lechleitner P, Dienstl F, Puschendorf B, Michel G (1994) Different time courses of cardiac contractile proteins after acute myocardial infarction. Clin Chim Acta 231:47–60. https://doi.org/10.1016/0009-8981(94)90253-4
Mao Y, Koga J-i, Tokutome M, Matoba T, Ikeda G, Nakano K, Egashira K (2017) Nanoparticle-mediated delivery of pitavastatin to monocytes/macrophages inhibits left ventricular remodeling after acute myocardial infarction by inhibiting monocyte-mediated inflammation. Int Heart J 58:615–623. https://doi.org/10.1536/ihj.16-457
McMullen JR, Jennings GL (2007) Differences between pathological and physiological cardiac hypertrophy: novel therapeutic strategies to treat heart failure. Clin Exp Pharmacol Physiol 34:255–262. https://doi.org/10.1111/j.1440-1681.2007.04585.x
Neri M, Fineschi V, Di Paolo M, Pomara C, Riezzo I, Turillazzi E, Cerretani D (2015) Cardiac oxidative stress and inflammatory cytokines response after myocardial infarction. Curr Vasc Pharmacol 13:26–36 Volume 13, Number 1, pp. 26–36(11)
Newton K, Dugger DL, Maltzman A, Greve JM, Hedehus M, Martin-McNulty B, Carano RAD, Cao TC, van Bruggen N, Bernstein L (2016) RIPK3 deficiency or catalytically inactive RIPK1 provides greater benefit than MLKL deficiency in mouse models of inflammation and tissue injury. Cell Death Differ 23:1565–1576. https://doi.org/10.1038/cdd.2016.46
Oerlemans MIFJ, Liu J, Arslan F, den Ouden K, van Middelaar BJ, Doevendans PA, Sluijter JPG (2012) Inhibition of RIP1-dependent necrosis prevents adverse cardiac remodeling after myocardial ischemia–reperfusion in vivo. Basic Res Cardiol 107(270):270. https://doi.org/10.1007/s00395-012-0270-8
Pazoki-Toroudi HR, Hesami A, Vahidi S, Sahebjam F, Seifi B, Djahanguiri B (2003) The preventive effect of captopril or enalapril on reperfusion injury of the kidney of rats is independent of angiotensin II AT1 receptors. Fundam Clin Pharmacol 17:595–598. https://doi.org/10.1046/j.1472-8206.2003.00188.x
Pazoki-Toroudi HR, Ajami M, Habibey R (2010) Pre-medication and renal pre-conditioning: a role for alprazolam, atropine, morphine and promethazine. Fundam Clin Pharmacol 24:189–198. https://doi.org/10.1111/j.1472-8206.2009.00743.x
Pazoki-Toroudi H, Amani H, Ajami M, Nabavi SF, Braidy N, Kasi PD, Nabavi SM (2016) Targeting mTOR signaling by polyphenols: a new therapeutic target for ageing. Ageing Res Rev 31:55–66. https://doi.org/10.1016/j.arr.2016.07.004
Puhl S-L, Müller A, Wagner M, Devaux Y, Böhm M, Wagner DR, Maack C (2015) Exercise attenuates inflammation and limits scar thinning after myocardial infarction in mice. Am J Phys Heart Circ Phys 309:H345–HH59. https://doi.org/10.1152/ajpheart.00683.2014
Rahimi M, Shekarforoush S, Asgari AR, Khoshbaten A, Rajabi H, Bazgir B, Mohammadi MT, Sobhani V, Shakibaee A (2015) The effect of high intensity interval training on cardioprotection against ischemia-reperfusion injury in wistar rats. EXCLI J 14:237 PMID: 26417361
Rakhshan K, Azizi Y, Naderi N, Afousi AG, Aboutaleb N (2018) ELABELA (ELA) peptide exerts Cardioprotection against myocardial infarction by targeting oxidative stress and the improvement of heart function. Int J Pept Res Ther 1–9. https://doi.org/10.1007/s10989-018-9707-8
Reiser PJ, Portman MA, Ning X-H, Moravec CS (2001) Human cardiac myosin heavy chain isoforms in fetal and failing adult atria and ventricles. Am J Phys Heart Circ Phys 280:H1814–H1H20. https://doi.org/10.1152/ajpheart.2001.280.4.H1814
Shindo R, Kakehashi H, Okumura K, Kumagai Y, Nakano H (2013) Critical contribution of oxidative stress to TNFα-induced necroptosis downstream of RIPK1 activation. Biochem Biophys Res Commun 436:212–216. https://doi.org/10.1016/j.bbrc.2013.05.075
Shulga N, Pastorino JG (2012) GRIM-19-mediated translocation of STAT3 to mitochondria is necessary for TNF-induced necroptosis. J Cell Sci 125:2995–3003. https://doi.org/10.1242/jcs.103093
Song L, Yang H, Wang H-X, Tian C, Liu Y, Zeng X-J, Gao E, Kang Y-M, Du J, Li H-H (2014) Inhibition of 12/15 lipoxygenase by baicalein reduces myocardial ischemia/reperfusion injury via modulation of multiple signaling pathways. Apoptosis 19:567–580. https://doi.org/10.1007/s10495-013-0946-z
Tao L, Bei Y, Lin S, Zhang H, Zhou Y, Jiang J, Chen P, Shen S, Xiao J, Li X (2015) Exercise training protects against acute myocardial infarction via improving myocardial energy metabolism and mitochondrial biogenesis. Cell Physiol Biochem 37:162–175. https://doi.org/10.1159/000430342
Ulbrich AZ, Angarten VG, Netto AS, Sties SW, Bündchen DC, de Mara LS, Cornelissen VA, de Carvalho T (2016) Comparative effects of high intensity interval training versus moderate intensity continuous training on quality of life in patients with heart failure: study protocol for a randomized controlled trial. Clinical Trials and Regulatory Science in Cardiology 13:21–28. https://doi.org/10.1161/CIRCULATIONAHA.114.011195
Vandenabeele P, Galluzzi L, Vanden Berghe T, Kroemer G (2010) Molecular mechanisms of necroptosis: an ordered cellular explosion. Nat Rev Mol Cell Biol 11:700–714. https://doi.org/10.1038/nrm2970
Wolff AM, Rasmussen TP, Wichern CR, Peterson MR, Stayton MM, Thomas DP (2017) Effects of pericardiectomy on training-and myocardial infarction-induced left ventricular hypertrophy, chamber dimensions and gene expression. Int J Sports Med 38:27–34. https://doi.org/10.1055/s-0042-115567
Zhang D-W, Shao J, Lin J, Zhang N, Lu B-J, Lin S-C, Dong M-Q, Han J (2009) RIP3, an energy metabolism regulator that switches TNF-induced cell death from apoptosis to necrosis. Science 325:332–336. https://doi.org/10.1126/science.1172308
Zhang T, Zhang Y, Cui M, Jin L, Wang Y, Lv F, Liu Y, Zheng W, Shang H, Zhang J (2016a) CaMKII is a RIP3 substrate mediating ischemia-and oxidative stress-induced myocardial necroptosis. Nat Med 22:175–182. https://doi.org/10.1038/nm.4017
Zhang T, Zhang Y, Cui M, Jin L, Wang Y, Lv F, Liu Y, Zheng W, Shang H, Zhang J (2016b) CaMKII is a RIP3 substrate mediating ischemia-and oxidative stress–induced myocardial necroptosis. Nat Med 22:175–182. https://doi.org/10.1038/nm.4017
Zhang L, Feng Q, Wang T (2018) Necrostatin-1 protects against Paraquat-induced cardiac contractile dysfunction via RIP1-RIP3-MLKL-dependent necroptosis pathway. Cardiovasc Toxicol 18(4):346–355. https://doi.org/10.1007/s12012-017-9441-z
Acknowledgements
Present work was funded by a research grant from Physiology Research Center in Iran University of Medical Science.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Rights and permissions
About this article
Cite this article
Ghardashi Afousi, A., Gaeini, A., Rakhshan, K. et al. Targeting necroptotic cell death pathway by high-intensity interval training (HIIT) decreases development of post-ischemic adverse remodelling after myocardial ischemia / reperfusion injury. J. Cell Commun. Signal. 13, 255–267 (2019). https://doi.org/10.1007/s12079-018-0481-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12079-018-0481-3