Abstract
The basic helix-loop-helix (bHLH) transcription factor family, which is widely prevalent in higher plants, plays an important role in regulating plant growth and development under various environmental factors. However, there is still no genome-wide analysis of the bHLH family conducted in mango (Mangifera indica L.). In this study, 212 MibHLH genes were identified in the mango genome by genome wide-analysis and they are unequally distributed across all the mango chromosomes. Based on the phylogenetic tree analysis, MibHLH proteins were divided into 26 subgroups. GO and protein-protein interaction network analyses showed bHLH protein family has multiple biological functions in mango. Several cis-elements related to hormone responses such as CGTCA-motif and TGACG-motif, ABRE, GARE-motif, TCA-element and TGA-element were identified in the upstream regions of MibHLH genes. The results provide some valuable information for exploring the functional roles of the MibHLH genes in mango.
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The authors appreciate the support of the Central Public-interest Scientific Institution BasalResearch Fund for Chinese Academy of Tropical Agricultural Sciences, grant number (No1630092020012)and the earmarked fund for the Belt and Road Tropical Project(BARTP-07).
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HS and NNWH designed the experiment. HS analyzed the results and prepared the manuscript. LT and RZ and revised the manuscript. All authorsreviewed and approved the final manuscript.
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Supplementary Information
Figure S1
Phylogenetic tree analysis, conserved motifs MibHLH protein family (A) the phylogenetic tree of bHLH proteins in mango (B) Ten motifs MibHLH proteins were identified by MEME are represented by colored boxes and consensus sequences. (PDF 279 kb)
Table S1
Primer sequence lists and nimble cloning method were used in this study. (DOCX 18 kb)
Table S2
MibHLH members, amino acids length, sub-molecular localization, isoelectric point (pI) and molecular weight. (XLSX 18 kb)
Table S3
Chromosomal position, gene annotation gene length, Exon/Intron of bHLH genes in mango genome. The positive (+) and negative (−) symbols following each gene represent forward and reverse orientations, respectively, on the chromosome. (XLSX 24 kb)
Table S4
Most abundant cis-regulatory elements were detected in promoter sites of MibHLH genes. (XLSX 246 kb)
Table S5
Predicted functions of the MibHLHs with the function of their homologs verified in Arabidopsis by phylogenetic analysis. (XLSX 18 kb)
Table S6
Summary information Of 212 MibHLH proteins was found in STRING database based on their homologs from Arabidopsis. (XLSX 27 kb)
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Salih, H., Tan, L. & Htet, N.N.W. Genome-Wide Identification, Characterization of bHLH Transcription Factors in Mango. Tropical Plant Biol. 14, 72–81 (2021). https://doi.org/10.1007/s12042-020-09277-w
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DOI: https://doi.org/10.1007/s12042-020-09277-w