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QTL Analysis for Root Protein in a Backcross Family of Cassava Derived from Manihot esculenta ssp flabellifolia

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Abstract

Root protein content of elite cassava is very low, largely due to breeder’s selection for other agronomic traits mainly fresh weight yield and disease resistance. Increased protein content in the root of cassava will improve its usefulness as a more complete food source in the developing world. An inter-specific F1 hybrid CW 198 - 11 was earlier developed at International Center for Tropical Agriculture (CIAT), Cali, Colombia by genetic crosses of OW 230 - 1 (FLA 441 - 5) and CW 30–65 (an inter-specific hybrid between an improved cassava variety SG 427 - 87 and an accession of Manihot esculenta ssp flabellifolia (MESCFLAX – 80)). The inter-specific cross was ‘backcrossed’, in the sense of another cross to cassava (MTAI – 8) to generate a B1P2 family with 225 progenies in which major quantitative trait loci (QTL) for root protein in the backcross population of cassava were identified. A linkage map from the female parent of the backcross population was used for QTL detection. A total of three QTL (protg.7, protg.13 and protg.23) controlling protein were identified in three different environments. One QTL was expressed across all three environments. These results demonstrated high broad sense heritability of 61.6% for protein over 2 years, in two different locations. The individual effects of alleles at these QTL explained from 15% to 25% of the phenotypic variance. The consistency of QTL controlling protein across environments reveals their potential for use in marker-assisted recurrent selection.

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Acknowledgements

This research work was supported by a pre-doctoral fellowship granted to CIAT from Rockefeller Foundation. Kirkhouse trust fund and Generation Challenge Programme supported my training in QTL mapping courses at NC State, summer courses.

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Authors and Affiliations

  1. Centro Internacional de Agricultura Tropical (CIAT), Km 17 Recta Cali - Palmira, Cali, Colombia

    O. Akinbo, J. Marín, C. Ospina, L. Santos, E. Barrera, J. Gutiérrez, E. Okogbenin & M. Fregene

  2. Department of Plant Sciences, University of the Free State, P.O. Box 339, Bloemfontein, South Africa

    O. Akinbo & M. T. Labuschagne

  3. National Root Crops Research Institute, Umudike, PMB 7006, Abia State, Nigeria

    O. Akinbo, F. Ewa & E. Okogbenin

  4. Donald Danforth Plant Science Center, 975 North Warson Road, St. Louis, MO, 63132, USA

    M. Fregene

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  1. O. Akinbo
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  9. E. Okogbenin
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Correspondence to O. Akinbo.

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Communicated by: Paul Moore

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Akinbo, O., Labuschagne, M.T., Marín, J. et al. QTL Analysis for Root Protein in a Backcross Family of Cassava Derived from Manihot esculenta ssp flabellifolia . Tropical Plant Biol. 5, 161–172 (2012). https://doi.org/10.1007/s12042-012-9095-8

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