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Consequence of cyclic pollen selection for heat tolerance on the performance of different generations in maize (Zea mays L.)

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Abstract

The reproductive stage in many crops, including maize, is very sensitive to heat stress and the genetic overlap between gametophytic and sporophytic phase gives an opportunity to select superior stress tolerant genotype at gametophytic stage. An attempt was made to evaluate the response of cyclic pollen selection in the F1 and F2 generations on the performance of F3 generation progenies for seed yield and yield contributing traits under natural heat stress conditions. In this direction three groups of F3 progenies, namely (i) pollen selection in F1 and F2 generations (GG), (ii) pollen selection only in F2 generation (CG), (iii) no pollen selection in F1 and F2 generations (CC) were screened for heat stress at Agricultural Research Station (ARS), Bheemarayanagudi. The GG progenies recorded significantly higher chlorophyll content, more number of pollen grains per anther and less pollen sterility compared to CG and CC group of progenies under heat stress. Further, the F4 progenies obtained through cyclic pollen selection (in F1, F2 and F3) were also tested for heat stress tolerance at seedling stage. The significant improvement for heat stress tolerance was recorded in F4 progenies derived through cyclic pollen selection as compared to control (no pollen selection for heat tolerance in any generation) F4 progenies. The results indicated that cyclic pollen selection in F1, F2 and F3 generations improved the heat stress tolerance of the progenies in the succeeding generations. To provide genetic evidence for the effect of pollen selection for heat tolerance, the control F2 (C) and selected F2 (G) populations were compared for the segregation of SSR markers. The selected F2 (G) population showed significant deviation from normal Mendelian ratio of 1:2:1 and showed skewness towards the alleles selected from male parent. The results provide strong evidence for an increase in the frequency of parental alleles in the progenies that impart heat stress tolerance.

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Acknowledgements

Authors acknowledge the DBT-FIST funded Department of Plant Biotechnology, UAS, GKVK Campus, Bengaluru, and Agriculture Research Station Bheemarayanagudi, UAS, Raichur for extending necessary facilities to carry out the research work.

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  1. Ashutosh Singh

    Present address: Centre of Advance Studies on Climate Change, RPCAU, Pusa, Samastipur, 848 125, India

Authors and Affiliations

  1. Department of Plant Biotechnology, UAS GKVK, Bengaluru, 560 065, India

    Ashutosh Singh, R. L. Ravikumar & Suresh H. Antre

  2. Department of Genetics and Plant Breeding, College of Agriculture Bheemarayanagudi, Shahapur, Yadigiri, 585 287, India

    P. H. Kuchanur

  3. Department of Genetics and Plant Breeding, UAS GKVK, Bengaluru, 560 065, India

    H. C. Lohithaswa

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  1. Ashutosh Singh
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Correspondence to Ashutosh Singh or R. L. Ravikumar.

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Corresponding editor: Durgadas P. Kasbekar

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Singh, A., Ravikumar, R.L., Antre, S.H. et al. Consequence of cyclic pollen selection for heat tolerance on the performance of different generations in maize (Zea mays L.). J Genet 101, 33 (2022). https://doi.org/10.1007/s12041-022-01373-y

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  • DOI: https://doi.org/10.1007/s12041-022-01373-y

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